254 
visible. 
Dr. W. Yorke. 
Auto-Agglutination of 
and the supernatant fluid removed (Digest solution). 
[Nov. 2, 
The xed cells were then quickly thrown down by centrifugalisation 
A 5-per-cent. suspension of red blood cells in normal saline solution was 
prepared from the following animals: Rabbit 1035 Gnfected with 7. brucev), 
normal Rabbit A, Donkey 2 (infected with 7’. rhodestense), normal Donkey A, 
Goat 1041 (infected with 7. rhodesiense), normal Goat A, and from a normal 
rat, guinea-pig, dog, horse, Macacus rhesus, Cercopithecus callitrichus, and 
human being. 
Table VI.—Agglutinating Action of a Solution Obtained by Digesting Auto- 
agglutinated Red Blood Cells with Normal Saline Solution at 40° C. 
Equal volumes of red cell suspension and defibrinated 
plasma or digest solution used. ‘Temperature of 
experiment 0° C. 
5 per cent. suspension of 
washed erythrocytes in 
normal saline solution. 
Untreated defibrinated plasma 
of Rabbit 1035 (infected with 
nagana), and solution 
obtained by digesting the 
auto-agglutinated red cells of 
the same animal with normal 
saline solution at 37° C. 
Result. 
Rabbit 1085 
with 7. brucet) 
(infected | 
Normal Rabbit A........ ... 
Donkey 2 (infected with 
T. rhodesiense) 
Normal Donkey A 
Goat 1041 (infected with 
T. rhodesiense) 
Normal Goat A 
eee ee rescore 
Cercopithecus callitrichus 
Human being 
| Defibrinated plasma 
ey 
| Digest solution 
Defibrinated plasma 
Digest'solution We sc senesdese ar: 
Defibrinated plasma 
Digestisolution j2265..csve.eccnee 
Defibrinated plasma 
Digest solutions Wer eee sees.2- 
Defibrinated plasma 
Digest solution® 201 acne 
Defibrinated plasma 
CoC ecce scores 
see ces POF eee 
Digest solution sn, porenie ems 
Defibrinated plasma 
Wirgest solution 2. cy. ce seas 
Defibrinated plasma 
Digest OlUbion do. -hecee sees 
Defibrinated plasma 
Digest isolution: 0.4. ..0.5. sees 
Defibrinated plasma 
Digest solwtion! 72... 6... sete 
Defibrinated plasma 
Digest solution 
re a 
Ce ee ec ay 
eee sree recces 
cea eee cores 
eee ree sececos 
CCP coer ern eesreosce 
Digest solution! 2254 .c..6.-+.-. ose 
Defibrinated plasma 
Wigestrsolupiong cece. c..-- <2 .| 
Defibrinated plasma 
eee PO eee e ene 
cee eer eee eee 
re ee ee ar 
Complete agglutination in 10 mins. 
Slight 
Complete 
No 
Complete 
No 
Complete 
9 10 =, 
” 10 ry) 
» 10, 
%9 10 _,, 
” 30 ,, 
9 15 2) 
” 45 ” 
” 106%; 
” 45 ,, 
” 10 ” 
” 45s, 
” 16, 
” 20 ;, 
” 1b) 
ns 20 ” 
” D3 
” LOT; 
” LO. 55 
” 45, 
” 1p) 5; 
” 30 5 
») LON 
49 15 ,, 
‘y 10 
” Los, 
N.B.—The four specimens of normal saline solution which had been used for washing the 
clumped red cells were also examined. 
agglutinin was found in these solutions. 
With the exception of occasional traces in the first, no 
