1910. | B. lactis aérogenes on Glucose and Mannitol. 275 
Composition and Optical Activity of Desiccated “Crude Glycol.” 
’ | | ; 
No. of sample.|Wt.taken.| CO. | HO.  C. js ame ae ea 
aqueous solution. 
| | 
| per cent. | per cent. 
C0 eee 0 1464 0°2886 0:°1448 52 °83 | 10 ‘99 +141 
100 ree 0 °2945 0°5770 | 0:°2925 53 °43 eT cit: +1°21 
1 OSS ee ona 0 °3190 0°6160 , 0°3164 52 °66 11 ‘09 
0:3682 | 0:°7147 | 0 -3669 52 94, ee OF e| + 0°92 
Molecular Weight of the “ Crude Glycol.’—A molecular weight determina- 
tion by Victor Meyer’s method gave the value 80°6. The temperature 
employed was 215°, and 0:0435 grm. of glycol gave 12°6 cc. of air at 15° 
and 776 mm. By Beckmann’s freezing-point method the value 80 was 
obtained— 
0°1386 grm. “glycol” in 22 c.c. aqueous solution gave A = 0:145 
0:2079 \ 3 e , Ai 030K, 
_ The formula weight of C,Hi902 = 90. 
Oxidation of the “ Crude Glycol.” 
(1) Oxidation in Aqueous Solution by Bromine in the Presence of Light. 
Formation of Diacetyl—To 0°9 grm. of crude glycol dissolved in 50 c.c. of 
water 3°25 grm. of bromine was added. The whole was well shaken from 
time to time and exposed to the light from an arc-lamp, care being taken not 
to allow the temperature of the mixture to rise to any considerable extent. 
After six hours a dilute aqueous solution of sulplur dioxide was added until 
the smell of bromine disappeared, and the mixture was saturated with 
sodium chloride. Distillation under a Young’s still-head of 15 discs yielded 
some drops of a yellow liquid which possessed the characteristic smell of 
diacetyl and dissolved in excess of water. 
Phenylosazone of Diacetyl—This was prepared in order to confirm the 
identification of this product of the oxidation of “crude glycol.” The 
solution of diacetyl obtained above was made up to 10 cc. with water, 
3 grm. of phenylhydrazine and 3 c.c. of 50 per cent. acetic acid added, and 
the whole kept at 100° for one hour. The phenylosazone deposited was 
recrystallised from alcohol and then melted at 241° to 243°. 
01147 grm. gave 20°6 cc. N (moist) at 18°5 and 765 mm. 
Found N = 20°8 per cent. Calculated for CigHigNs, N = 21:05 per cent. 
The osotetrazone prepared from this by oxidation’ with potassium 
bichromate and acetic acid melted at 153°. The needles of this substance, 
VO x Ct, —B, Y 
