1910. ] B. lactis aérogenes on Glucose and Mannitol. 285 
The acetylmethylcarbinol was identified by conversion into the osazone of 
diacetyl. 
The relative amounts produced anaérobically and aérobically from 2: 3- 
22-4 
36 
nearly the same ratio as that found to exist between the corresponding 
amounts from glucose, 1:5°6. The absolute amounts were :— 
butanediol were therefore as 1: = 1 : 6:2, which is, curiously enough, 
III. THe Action oF B. lactis aérogenes ON FRUCTOSE. 
One litre of medium was made up to contain 1 per cent. of peptone, 
15 per cent. calcium carbonate, and 5:0 per cent. of fructose. The period 
of incubation at 37° was 37 days. After filtration the volume was 963 c.c., 
and from this 750 c.c. were distilled. The distillates readily reduced 
Fehling’s solution in the cold, and when extracted with ether after saturation 
with potassium carbonate a liquid was obtained which gave with phenyl- 
hydrazine acetate an osazone melting at 240° C. It was therefore concluded 
that acetylmethylearbinol was present. The residue was treated in the 
manner described for glucose, and the weight of crude glycol obtained was 
3°2 grms. The value of [«]p was 1°32 in 10-per-cent. solution in dry ether. 
During the course of this investigation the following substances, in addition 
to those described, were prepared for purposes of comparison :— 
1. Diphenylurethane of 1 :3-butanediol was obtained from the diol prepared 
by the reduction of aldol. It crystallises readily from alcohol in colourless 
prisms, melting at 122° to 123° C. ; 
0:2763 grm. gave 20:2 c.c. N at 14°°8 and 768 mm. 
Found. Calculated for C,,H,,N,0,. 
ING ot erer St 8°53 
2. Diphenylurethane of 1:2 Propanediol—tThe glycol from which it was 
prepared boiled at 185° to 195° at ordinary pressure. The diphenylurethane 
is readily soluble in hot alcohol, from which it is for the most part pre- 
cipitated in colourless silky needles on cooling. These melt at 152°5 
to 153° 5. 
0:2571 grm. gave 0°6138 grm. CO2 and 01306 grm. H20. 
02581 grm. ,, 19°7 c.c. N (moist) at 770 mm. and 20° C. 
