1910.] Proteins into Fats during the Ripening of Cheese. 303 
Hxpervmental Part. 
6874 grm. of a Cheddar cheese, about four years old, were extracted with 
ether in a Soxhlet apparatus. The ethereal solution was evaporated to 
dryness, the residue dissolved in ether and precipitated with acetone, the 
precipitate collected, and the process repeated four times. The different 
ether and acetone solutions were collected and examined separately. 
The precipitate (175 grm.), which contained traces of nitrogen, was 
redissolved in ether and once more precipitated with acetone, but only the 
precipitate formed at once was collected. The product thus obtained was free 
from nitrogen. When dissolved in warm chloroform it separated on cooling 
in feather-like crystalline needles, melting at 139° to 140° (cholesterol melts 
at 147°), and gave all reactions for cholesterol. But in order to further 
confirm the presence of this substance, the benzoyl derivative was prepared ; 
this crystallised from alcohol in octahedra, which melted at 176° to 177°, and 
gave on analysis the following data :— 
01527 grm. gave 04654 grm. CO: and 0:1399 grm. H,0. 
C.;H,,;0°C;H;0 requires C = 83°20, H = 10°20. 
Found @= 83°12, H = 10°18. 
All the acetone-ether solutions were collected and evaporated to dryness, 
_ and the residue treated in the cold with sodium carbonate and saturated with 
carbon dioxide. When carefully acidified with dilute sulphuric acid the 
filtrate gave a white precipitate which contained nitrogen. The melting 
point of this substance, crystallised from either benzene or alcohol and 
methylated spirit, was between 162° and 229°, showing that it was not pure. 
It was purified by dissolving 1 grm. of the material in 40 c.c. of absolute 
alcohol and adding 5 c.c. of boiling benzene to the solution. On cooling, 
well-defined needles were formed, which melted at 246° to 251°, and gave the 
following data on analysis :— 
02731 grm. gave 0°5116 grm. CO: and 0°2334 grm. H20. 
0:1881 . 19°5 cc. Ne (19°, 758 mm.). 
C53H1,02N (aminovaleric acid) requires C = 51°28, H = 9°40, N = 11:95. 
Found C = 51:09, H = 9°36, N = 12:07. 
The benzoyl derivative prepared by Fischer’s method formed small prismatic 
needles (m. p. 173°—179°), which gave the following data on analysis :-— 
01872 grm. gave 0°4556 germ. COz2 and 0°1139 grin. H20. 
01531 “ 8:5 c.c. Ng (16°, 756 mm.). 
Ci2Hi;03N requires C = 65°16, H = 6°78, N = 6°38. 
Found C = 64:93, H = 6°81, N = 6755. 
