312 Col. Sir D. Bruce and others. Antelopeasa [Nov. 9, 
would be almost impossible to check, owing to the difficult nature of the 
forested and elephant-grass country which in so many parts borders the 
Uganda shores of the Lake. Game laws would require modification, and the 
destruction of the game in the neighbourhood of the lakes and palpalis- 
frequented rivers would become a factor in the control of Sleeping Sickness. 
To answer the above queries the following experiments were devised and 
carried out :— 
1. Can Antelope be Infected with Sleeping Sickness by the. Bites of Laboratory- 
bred and Laboratory-infected Glossina palpalis ? 
Eleven buck in all were employed in this experiment. Four bush-buck 
(Tragelaphus sylvatica ?), six reed-buck (Cervicapra arundinacea), and one 
water-buck (Cobus defassa). Other buck, such as “oribi” (Cephalophus 
grimmt) and “ entalaganya” (Cephalophus equatorialis), were obtained by the 
Commission, but did not survive long in captivity. 
As each buck arrived its blood was usually injected, subcutaneously, into 
monkeys or rats, to ascertain if the blood was naturally infected with trypano- 
somes. The method then adopted in each case to infect the antelope with 
Sleeping Sickness was as follows: A cage of clean™ laboratory-bred Glossina 
palpalis was fed on a monkey known to be infected with a human strain of 
Trypanosoma gambiense. This cage of artificially-infected flies was then fed 
on healthy animals until one of the animals (monkeys) became infected with 
Sleeping Sickness. Having proved that the flies were infective, the cage of 
flies was then fed, about five minutes daily, for several days, on one or other 
of the buck. The buck’s blood was then examined daily for trypanosomes, 
and was further tested for Sleeping Sickness by its inoculation into monkeys 
or rats. 
The experiments follow in full detail. 
Experiment 2328. Bush-buck. 
This bush-buck was fed on for five days (March 24, 25, 26, 28, and 29, 1910) by 
laboratory-bred Glossina palpalis which were known to be infected with a human strain 
of Trypanosoma gambiense. 
On April 8, 15 days after the first feed of the infected flies on this buck, 5 cc. of the 
buck’s blood were injected, subcutaneously, into a healthy monkey. 
April 19, monkey showed Trypanosoma gambiense in its blood. The trypanosomes 
were verified by examination in fresh and stained preparations of the blood. 
Result.—Positive. 
Remarks.—The preliminary inoculation of the blood into susceptible animals, to 
* The word “clean,” applied here and throughout this paper to laboratory-bred 
Glossina palpalis, means flies which have newly hatched out from pup in the laboratory 
and have never fed. 
