314 Col. Sir D. Bruce and others. Antelopeasa_ [Nov. 9, 
Experiment 2371. Bush-buck. 
On April 138, 1910, 5 c.c. of this buck’s blood were injected, subcutaneously, into a. 
normal monkey, to ascertain if buck’s blood naturally harboured trypanosomes. This 
monkey’s blood was examined bi-weekly for a month. Monkey remained healthy. 
Buck was fed on for eight days (April 22 and 23, and from April 25 to 30, 1910, 
inclusive) by laboratory-bred Glossina palpalis known to be infected with a human strain 
of Trypanosoma gambiense. 
On May 4, the 12th day after the infected flies’ first feed on the buck, Trypanosoma 
gambiense appeared in the buck’s blood in fair numbers. | 
On May 5, trypanosomes were numerous, and 2 ¢.c. of the buck’s blood were injected, 
subcutaneously, into a normal monkey. On May 13 this monkey showed Trypanosoma 
gambiense in its blood. 
Result.—Positive. 
Remarks.—Buck was free from trypanosomes inoculable into a monkey on its arrival at. 
the laboratory. Trypanosoma gambiense appeared in buck’s blood for the first time, in 
scanty numbers, on May 3, 1910, and again in fair numbers on May 4; thereafter no 
trypanosomes were seen up to August 5, 1910, though the blood was almost daily 
examined. 
Experiment 2378. Water-buck. 
On April 13, 1910, 5 c.c. of this buck’s blood were injected, subcutaneously, into a 
normal monkey, to ascertain if the buck naturally harboured trypanosomes in its blood.. 
This monkey was examined bi-weekly for one month ; monkey remained healthy. 
Buck was fed on for eight days (Aprii 22, 23, 25 to 30, inclusive) by laboratory-bred' 
Glossina palpalis known to be infected with a human strain of Trypanosoma gambiense.. 
Trypanosoma gambiense was never seen in this buck’s blood, though examined for almost: 
daily from April 22 to August 5, 1910. 
On May 5,5 c.c. of the buck’s blood were injected, subcutaneously, into a normal 
monkey, an interval of 13 days having elapsed since first feed of the infected flies. This 
monkey showed Trypanosoma gambiense in its blood on May 13, 1910. 
Result.— Positive. 
Remarks.—Buck was free from trypanosomes inoculable into a monkey on its arrival at 
the laboratory. Though Trypanosoma gambiense never appeared in the buck’s blood, yet 
a positive result was obtained on its inoculation into a monkey, and, as will be seen below 
(Table IL), clean laboratory-fed flies fed on this buck for several days became infected with 
Trypanosoma gambiense. 
Experiment 2427. Reed-buck. 
On May 4, 1910, 1 cc. of this buck’s blood was injected, subcutaneously, into a normal 
white rat. This rat, examined bi-weekly, for one month, remained healthy. 
Buck was fed on for six days (May 2 to 7, inclusive) by laboratory-bred Glossina 
palpalis known to be infected with a human strain of Trypanosoma gambiense. 
On May 9, the seventh day after infected flies’ first feed on the buck, 7’rypanosoma 
gambiense appeared in scanty numbers in its blood for the first time. One cubic centi- 
metre of the blood was then injected, subcutaneously, into a normal white rat. On 
May 16, Trypanosoma gambiense appeared in this rat’s blood. 
Result.—Positive. 
Remarks.—On its arrival at the laboratory the buck was free from trypanosomes 
inoculable into a rat. A few Trypanosoma gambiense appeared for the first time on 
May 9, 1910, in buck’s blood ; they were present in fair numbers on the 10th, scanty 
