320 
Experiment 2501.—To ascertain if Laboratory-Bred 
Col. Sir D. Bruce and others, 
Antelope asa _ [Nov. 9, 
Glossina palpalis 
become Infective when Fed on Antelope whose Blood contains 
Trypanosoma gambiense. 
| 
Date. 
| 
| 1910. 
| May 23—28 ...' 
| 
| OY GIO, Ay deen 
i 32 30—31 
Hecliumie sl eeneeen oe 
» 2—Jduly7 
| Dal ye Shrsser eocnes 
| 
{ 
or MOR cumaeeese | 
Day of 
experi- 
ment. 
Procedure. 
Flies fed 5 minutes daily on 
Bush-buck 2828. 
Flies starved. 
Flies again fed 5 minutes daily 
on Buck 2328. 
Flies starved. 
Flies fed 5 minutes daily on 
normal Monkey 2517. 
Flies starved, as Monkey 2517 
shows Z. gambiense in its 
blood. 
The 57 surviving G. palpalis 
dissected; 20°3 per cent. of 
these flies showed heavy in- 
testinal infection with flagel- 
lates (7. gambiense). 
Result. 
~ 
Remarks. 
| 
100 flies used. 
July 8, Monkey 2517 
shows 7’. gambiense in its 
blood to-day. Allowing 
7 days for incubation of 
T. gambiense in monkey’s 
blood, then the G. pai- 
palis became infective 
on the 39th day after 
their first infected feed 
on Buck 2328. 
Remarks.—This is the experiment referred to above, where the blood of a buck was capable of 
infecting clean laboratory-bred flies 55 days after last feed of infected flies on the buck. 
Experiment 2499.—To ascertain if Laboratory-Bred Glossina palpalis 
become Infective when Fed on Antelope whose Blood contains. 
Trypanosoma gambrense. 
Date. 
Day of 
ment. 
experl- 
Procedure. 
1910. 
Mayi28=-28)...., 
eoeeece 
eeeocceeg see 
eceoee 
eee ccc ves eee 
July 13 and fol- 
lowing days. 
Ee ———————E 
51 
| Flies fed 5 minutes daily on 
Bush-buck 2372. 
Flies starved. 
Flies again fed 5 minutes 
| daily on Buck 2372. 
Flies starved. 
| Flies fed on Cock 2518. 
| Flies starved. 
13—July 12; 21—50 Flies fed 5 minutes daily on 
' clean Monkey 2552. 
| Flies allowed to die. Not 
| dissected as experiment 
negative. 
Result. 
Remarks. 
100 flies used. 
Monkey 2552 was exam- | 
ined bi-weekly from 
June 13 to August 12. 
ft remained healthy. 
The surviving G. pal- 
palis were not dissected 
as experiment was nega- 
tive. 
