324 Col. Sir D. Bruce and others. Antelopeasa_ [Nov. 9, 
together, it is seen that a total of 1,102 flies were examined. Of these, 
119 flies—54 male and 65 female—were infected with developmental forms 
of Trypanosoma gambiense—that is, 10°8 flies in every hundred became 
infected, the sexes being about equally imphecated. The highest percentage 
of infected flies in any one of the positive experiments was 21 per cent., 
in Experiment 2501, and the lowest was 1°3 per cent., in Experiment 2592. 
If to the total of 1,102 flies dissected in the positive experiments be added 
all the Glossina used in all the negative experiments, we get a total of 
1,722 flies employed, and 6°9 flies in every hundred infected. 
It is perhaps worth noting that a diet for the fly of antelope, fowl, and 
monkey blood gave a higher percentage of negative results and a lower 
percentage of flies infected than a diet of antelope and monkey blood only. 
This was quite unexpected, for the Commission, as a result of many experi- 
ments and considerable experience, were of the opinion that fowl’s blood 
assisted the development of 7rypanosoma gambiense in Glossina palpalrs. 
4. How does Sleeping Sickness Affect the Health of Antelope ? 
This point is of considerable importance. If the disease killed the 
antelope within a short time of infection, or even if it seriously affected 
their health so as to render them incapable or unwilling to move about 
freely, the facts detailed above would lose some part of their practical 
value. 
The word “health” is not used here in a technical sense—that is to say, 
the health was not estimated by a series of blood counts and temperature . 
charts. Interesting as such observations would have been, the Commission 
regret they were too short-handed and too much pressed by other work to 
carry them out. 
Careful observations were made daily to answer the following questions :— 
Did the infected antelope during the time they were under observation 
appear sick? Did they become emaciated? Was there loss of health and 
strength? Were there corneal opacities, edematous swellings, conjunctival 
discharges, or staring coats? These questions may at once be answered in 
the negative, except in the case of Reed-buck 2445, which wiil be referred 
to later below. 
When the antelope were brought to the laboratory by the native hunters 
they invariably suffered from exhaustion, due probably to a combination of | 
causes, such as fright, confinement for two to four days in cages too small 
to allow free movement, insufficient water and food, and the rough usage 
undergone when being caught. As a result of these unfavourable con- 
