326 Col. Sir D. Bruce and others. Antelope asa [Nov. 9, 
natives would be required. Large numbers of clean laboratory-bred Glossina 
must be available, many normal susceptible animals must be in readiness, 
and, at the same time, be so situated that they cannot be bitten by the - 
possibly infected wild flies in the neighbourhood. The laboratory work in 
hand may have to be abandoned for the time being, and some 50 or 60 buck 
must be captured or killed before reliable information is forthcoming. The 
sun is hot, the country very difficult, and the exposure to the bites of the 
fly very great. 
It may be said at once that the Commission were only able to shoot two 
buck on the Lake-shore. The blood of these gave negative results for 
Trypanosoma gambiense when injected into susceptible animals. One of them, 
however, gave a positive result for Zrypanosoma vivax. This trypanosome 
was proved by the Commission* to be not uncommonly carried by wild Lake- 
shore Glossina palpalis. It is therefore not unreasonable to suppose that at. 
least one of the buck shot had been fed on by the Lake-shore fly. 
It was recognised at length that occasional week-end shoots by a member 
of the Commission were quite inadequate. The assistance of the Acting- 
Governor of Uganda, 8. C. Tomkins, Esq., C.M.G., the Acting Principal 
Medical Officer, Dr. C. Wiggins, of the Uganda Medical Staff, the Provincial 
Commissioners of Kampala, F. A. Knowles, Esq., C.M.G., and L. H. Cubitt, Esq., 
and of Sir Apolo Kagwa, K.C.M.G., the Prime Minister, was then sought to 
aid in organising a series of large drives of wild game in the fly-area in the 
neighbourhood of the laboratory. We are greatly indebted to these gentle- 
men for their ready efforts on our behalf, which resulted in a large drive 
being organised. Unfortunately, the Commission were ordered to leave the 
country before the drive could take place. The work on these lines, however, 
is being continued by Dr. R. van Someren, of the Uganda Medical Staff, and 
Captain A. D. Fraser, Royal Army Medical Corps, who were instructed to 
take over the laboratory work. 
This very difficult question, therefore, still avait its answer. 
Conclusions. 
1. Water-buck, bush-buck, and reed-buck can readily be infected with a 
human strain of the trypanosome of Sleeping Sickness by the bites of infected 
ae paulpalrs. 
. One exposure to the bites of infected flies is sufficient to infect; an. 
Dae with the virus of Sleeping Sickness. 
3. Though the blood of an antelope may be proved to be infected with 
* Vide ‘Roy. Soc. Proc.,’ B, 1910, vol. 82, pp. 68—66. 
