330 Col. Sir D. Bruce and others. Domestic Fowl of [Nov. 12, 
Experiment 1915 a. 
Day of | | | | 
Date. experi- | Procedure. Result. | Remarks. 
ment. 
1909. 
| Nov. 22—25 ...... 1—3 | Fhes fed on 7. gambiense- | 250 laboratory-bred G. 
infected monkey. palpalis used. 
» 26—Dec. 31 | .4—89 | Flies fed on Cock 1926. — T. gambiense-infected flies 
| found on Dec. 13, 14, 
| 1910. 16, 17, 20, and 27. 
Jan. 1—10.......... 40—49 | Flies fed on normal monkey, | 100 flies of this experi- 
| Experiment 1999, to test | | ment survived to 50th 
their infectivity. | day arid were used for 
| | Experiment 19150. 
ame BE cos eee 50 Monkey, Experiment 1999, + Allowing 7 days for the 
examined ; blood showed | incubation of 7. gambi- 
T. gambiense. | ense in this monkey, it 
| is seen that the flies 
infected it on Jan. 4— 
| that is, 4 days after 
feeding on fowl. 
Remarks.—On December 17, 1909, 1 c.c., and on December 22, 3 c.c. of the blood of cock, 
Experiment 1926, were injected into a normal monkey, Experiment 1954. This monkey 
remained healthy. On January 10, 1910, the cock was killed, 10 c.c. of its heart’s blood were 
centrifuged, and the “buffy layer” carefully examined for Trypanosoma gambiense, with 
negative results. The whole of the 10 c.c. of blood obtained were then injected into two 
monkeys: 5 c.c, into monkey, Experiment 1954, and 5 c.c. into monkey, Experiment 2054. The 
blood of these two animals was examined frequently for a month after these injections. No 
trypanosomes were ever found and the monkeys remained healthy. 
Experiment 2081. 
l 
Day of 
Date. experi- Procedure. Result. | Remarks. 
ment. 
1910. 
‘Jan. 9—14 ..., 1—5 | Fles fed on TZ. gambiense- 120 laboratory-bred G. 
infected monkey. | palpalis used. 
» 15—Feb. 2; 6—24 | Flies fed on cock, Experiment , — Feb. 15, monkey, Experi- | 
2074. ment 2135, showed 7’ | 
Feb. 3—7...... 25—29 | Flies fed on normal monkey,; + gambiense in its blood. 
Experiment 2135, to see if Feb. 22, monkey, Experi- 
flies are infective. ment 2178, showed 7. 
5 8—14 ...| 830—86.) Flies again fed on cock, Ex- — gambiense in its blood. 
periment 2074. Fow!, Experiment 2074, 
,, 15—19 ...| 37—41 | Flies fed on normal monkey, + has therefore been fed 
Experiment 2178, to see if on by infective G. pal- 
flies still infective. palis. 
Remarks.—Cock, Experiment 2074, was killed on March 16, 1910; 10 c.c. of its heart’s blood 
were tuken and centrifuged and carefully examined for Trypanosoma gambiense, with negative 
results. ‘The 10 c.c. were then injected, subcutaneously, into normal monkey, Experiment 2301. 
This monkey was examined bi-weekly up to April 15, 1910. No Trypanosoma gumbiense were 
ever seen, and the monkey remained healthy. 
a 
