1910.| Uganda as a Reservoir of Virus of Sleeping Sickness. 331 
The 13 experiments carried out on these lines are now given in tabular 
form, in Table I below :— 
Table I. 
| a 
N | No. of | 
Feviori.| No.of | No.of | infected flies | poi ing as 
on expert | flies used. fowl fed on. | found during pela ed 
ment. | | experiment, | 
: ei A 
19lia 250 Expt. 1926 7 Negative | Proved infective flies fed 
| on this fowl. 
19156 100 ee yt 2009 1 - Proved infective flies fed | 
ie | | on this fowl. 
1915 ¢ 50 eee 2 So — | se | Doubtful whether infected 
| | | flies fed on this fowl. | 
1918 100 Pat ian lO — 4 Proved infective flies fed | 
| | | on this fowl. 
2081 120 ae 2004 2 | y Proved infective flies fed 
| ; on this fowl. 
2082 100 re eS 12 | = Proved infective flies fed 
| | on this fowl. 
2208 100 » 2209 0 :) Proved infective flies fed 
| on fowl, but no flies 
| dissected. 
2325 160 | 45 2884 2 % Proved infective flies fed 
| | on fowl. 
2362 110 hy lady 2868 7 . | Proved infective flies fed 
| on fowl. 
2365 300 i, | 2a60 O | ; | Flies not dissected, but 
| | were proved infective. 
2366 150 » 2364 0 - Flies not dissected, but 
| | were proved infective. 
2390 180 i 2367 ii | te | Proved infective flies fed 
| | on fowl, 
2395 100 5, 2096 1 if Proved infective flies fed 
on fowl. 
| 
Remarks.—In these experiments it is seen that 1820 laboratory-bred and laboratory-infected 
Glossina palpalis were fed on 13 fowls, with uniformly negative results. With one exception, 
‘Experiment 1915 c¢, the flies were proved to be infective by feeding them on animals susceptible 
to Trypanosoma gambiense infection. That the fowls had not become infected with Trypanosoma 
gambiense was proved by carrying out the three tests given above, under headings a, 6, and c. 
In Experiment 2325, the liver, spleen and bone-marrow of Fowl 2334 were also examined for 
‘trypanosomes, and 10 c.c. of the pooled pulp injected into a normal monkey. This monkey 
remained healthy. 
A further series of experiments bearing on this question was carried out. 
These experiments are not so complete. The procedure was as follows :— 
A varying number of laboratory-bred Glossina palpalis were fed for several 
days on Trypanosoma gambiense-infected antelope. The infected flies were then 
fed for varying periods on fowls. The fowls were then killed and 2 cc. of 
their blood were injected into a normal monkey. The monkey’s blood was 
examined for 7rypanosonua gambiense bi-weekly for a month after the injection 
of the fowl’s blood. The experiments are given in tabular form. 
