1910. | as Carriers of Trypanosoma pecorum. 357 
Juscomarginatus contained parasites. The three infected flies all contained 
flagellates in the hind-gut, rectum, and proctodeum, which resembled 
Types 1 and 2 mentioned above. 
Type 3, Elongated, Flagellated Parasites. 
One fly, a Tabanus secedens, contained many flagellates, of a different 
variety, in the fore-gut, mid-gut, and fore-part of hind-gut. In the fresh 
preparation this third type of flagellate was motile, and possessed a poorly 
developed undulating membrane, which was thrown into short folds. The 
movements of the parasite were active, but not translatory to any great 
extent. The accompanying drawing (Plate 16, figs. 15 to 24) so clearly show 
the structure of this type in the stained state that further description is 
unnecessary. 
Type 3 occurs principally in the thick-walled mid-gut of the fly and, to a 
lesser extent, in the fore-gut and fore-part of the hind-gut. Types 1 and 2 
occur only in the hind-gut, rectum, and proctodzum of the fly. 
Inoculation Eaperiments carried out with these Parasites. 
Expt. 2404. 
April 23, 1910.—White rat inoculated, intra-peritoneally, with contents of hind-gut, 
rectum, and proctodzeum of a wild Tabanus secedens. This fly had been in fly-compart- 
ment of kraal ; it was heavily infected with Type 1 of the parasite. 
Result.—Negative. Rat examined for one month after injection. 
Expt. 2405. 
April 23, 1910.—White rat inoculated, intra-peritoneally, with contents of fore-gut and 
mid-gut of a wild Tabanus secedens. This fly had been in kraal, and its fore-gut and 
mid-gut were swarming with Type 3 of the parasite in an active state. 
Result.—Negative. Rat examined for one month after the injection. 
Expt. 2411. 
April 27, 1910.--White rat inoculated, intra-peritoneally, with hind-gut, rectum, and 
proctodeum of a wild Tabanus secedens. This fly had never been in kraal. It was 
heavily infected with Type 2 of the parasite. 
Result.—Negative. Rat examined for one month after injection. 
Expt. 2412. 
@ 
April 27, 1910.—White rat inoculated, intra-peritoneally, with hind-gut, rectum, and 
proctodzum of a wild Tabanus secedens. This fly had never been in kraal ; it was heavily 
infected with Type 1 of the parasite. 
esult.—Negative. Rat examined for one month after injection. 
