364 
exhibited in Table IV, that is to say, a still further 
of the hemolysis took place,* no laking being 
Dr. J. O. W. Barratt. 
| Deen 
reduction in the amount 
observed at the end of 
Table I1I.—Complement Fixation by Mouse Tumour Extract in the presence 
of inactivated normal Mouse Serum. 
Net ae” 
of oe 
tube. me 
serum. 
| C.c. 
1 0:001 
2 0 :003 
3 0 :009 
4 0 :027 
5 | 0:081 
Inac- 
tivated 
normal 
serum of 
mouse. 
Antigen 
(tumour 
extract). | 
c.c. 
0°03 
0°03 
0°03 
0 ‘03 
0°03 
The contents of each tube, after 
standing at 37° C. for 14 hours, were added to sensibilised red cells 
obtained from 0°02 cc. of a 24-per-cent. suspension of the red cells of 
the rabbit, and the mixture kept, with occasional shaking, at 37° C. for a 
further period of 14 hours. 
Degree of hemolysis at the end of— 
30 mins. 
Not 
recognisable 
99 
Partial 
Complete 
60 mins. 
Not — 
recognisable 
Slight 
Complete 
99 
90 mins. 
Not 
recognisable 
Slight 
Complete 
39 
18 hours 
(at room 
temperature). 
Not 
recognisable 
Slight 
Complete 
9 
Table [1V.—Complement Fixation by Mouse Tumour Extract in presence of 
inactivated Serum of Mouse with Tumour. 
The contents of each tube, 
after standing at 37° C. for 14 hours, were added to sensibilised red cells 
obtained from 0°02 c.c. of a 24-per-cent. suspension of the red cells of 
the rabbit, and the mixture kept, with occcasional shaking, at 37° C. for 
a further period of 14 hours. 
Inaec- 
a Active tivated : | 
No. | oman | serum of | “Dtigen | 
of (tumour 
blood mouse 
tube. . extract). 
serum. with 
tumour. 
C.c. c.c. c.c. 
1 | 0:°001 0°03 0:03 
2 0::003 0-03 0:03 
3 0 :009 0:03 0:03 
4 0 :027 0:03 0:03 
5 | 0:081 0 ‘03 0-03 
| 
| 
Degree of hemolysis at the end of — 
30 nine, 
Not 
recognisable 
Nearly 
complete 
60 mins. 
Not 
recognisable 
Slight 
Complete 
| 
90 mins. 
Not 
recognisable 
9 
Nearly 
complete 
Complete 
18 hours 
(at room 
temperature). 
Not 
recognisable 
29 
Complete 
3) 
‘ 
* In each comparative test the two series represented in Tables III and IV were 
performed simultaneously, the same active normal serum being used in both series. 
