1910. ] Complement Deviation in Mouse Carcinoma. 367 
Table V.mHxmolytic Action of Mouse Serum upon Red Blood Cells derived 
from various Sources. Temperature of experiment, 37° C. 
Ze Per | Active Hemolysis at the end of 90 minutes. 
No. cent. ae 2 Sein LS ted 
of |suspension| “5¢ | | | 
tube.| of red | Red cells of _ Red cells of | Red cells | Red cells of | Red cells of 
blood cells.) ™°S° | man. goat. of rabbit. | guinea-pig. rat. 
c.c. lee cic: | | 
1 0-02 0-001 Not | Not | Partial Not | Not 
| | _ recognisable | recognisable recognisable , recognisable 
pean O1 02 0-003 | Exceedingly | Bs | Nearly si | Exceedingly 
slight | complete slight 
3 0 02 0-009 ‘3 _Exceedingly | Complete | Exceedingly am 
| slight | | slight 
4 | 0°02 0-027 | " | - ook. : | a 
5 0 :02 0:054 | Shght Shght s Slight Slight 
It therefore became necessary to obtain a supply of mouse serum 
sufficiently rich in hemolysin for use, after inactivation, in sensibilising the 
red blood cells of the rabbit. It was also necessary that the amount of 
serum obtained should be enough for an extended series of observations. To 
this end 23 mice received intraperitoneally injections of the red blood cells 
of the rabbit in the following amounts :— 
0-005 c.c. of red cells on the 1st day. 
0°01 ” ” ord »” 
0:02 5 ‘ 4th ,, 
Nineteen of these mice still surviving were killed with ether on the 
18th day, and immediately after death blood removed with aseptic 
precautions from the right ventricle of each by means of a glass pipette. 
The sera thus obtained were mixed together and the hemolytic power of 
the mixture upon the red blood cells of the rabbit determined. The 
results obtained are given in Table VI. The maximum amount of serum 
required to completely hamolyse, within 90 minutes, 0°02 cc. of a 
4-per-cent. suspension of the red cells of the rabbit was found to be about 
0:009 cc. In sensibilising the red blood cells of the rabbit with this serum, 
after inactivation, when testing for complement in the experiments described 
below, twice this minimum amount was employed, the time allowed for 
sensibilising at 37° C. being one hour. 
The method pursued in the further course of these experiments was so 
similar to that followed in the preceding section that no additional descrip- 
tion of technique employed is required. 
Laupervmental Results—The action of fresh* mouse serum on the sensibilised 
* That is to say, obtained within 30 to 60 minutes of the death of the animal. . 
