1910. | Complement Deviation in Mouse Carcinoma. 371 
serum to be employed would of course be the same as that of the inactivated 
normal mouse serum. ‘The amount of active normal mouse serum employed 
as source of complement ranged from 0:02 c.c. to 0:06 ce. 
Table IX.—Complement Fixation by Amboceptor of inactivated Normal 
Mouse Serum in presence of an excess of Tumour Extract. The contents 
of each tube, after standing at 37° C. for 1 hour, were added to 
sensibilised red cells obtained from 0°02 c.c. of a 24-per-cent. suspension 
of the red cells of the rabbit, and the mixture kept, with occasional 
shaking, at 37° C. for a further period of two hours. 
Active ISD eae Inactivated Hemolysis at end of-— 
No. | 5a: cent. ree Mouse 
of Ge Ali solution eae tumour ae 
tube.) of sodium BA extract. 
Th ae chloride. - ee 30 mins. 120 mins. | 
xc: C.c. G:C, Cie: | 
1 0-010 0 °075 — — Slight Nearly complete 
2 0 °020 0 :065 — — 5 Complete 
3 0 :030 0 °055 — — Partial _ 
4, 0-010 0-030 0 “Gos 0°020 | Not recognisable | Not recognisable 
5 0-010 0-025 0-010 0 -020 3 
6 0 ‘010 0-015 0 020 0 °020 re % 
t 0-010 0 045 0-010 0°005 | Not recognisable Slight 
8 0-010 0 -040 0-010 0-010 me Not recognisable 
5 0-010 0 030 0-010 0-020 a 
9 0-030 0-040 0 -020 0-095 Very slight Complete 
10 0-030 0-035 0-020 0-010 a Nearly complete 
11 | 0-030 0-025 0 020 0-020 a 2 
A number of experiments was now carried out, in which the degree of 
complement fixation by inactivated normal mouse serum was compared 
with that of inactivated tumour mouse serum in presence of an excess of 
antigen. In Table X, a series of observations of this character is given. 
It is seen that when a comparison is made between the tubes in which 
inactivated tumour mouse serum was employed (7 to 9, 10 to 12, 13 to 
15), and those in which inactivated normal mouse serum was used (4 to 
6), the amount of complement fixation was distinctly greater in the former 
than in the latter. 
but it is nevertheless unmistakable. 
It is true that the difference in fixation is not profound, 
lt must also be remembered that the 
amboceptor corresponding to the antigen is partly contained in the active 
normal serum employed, and that, in consequence of this, the difference 
between the two inactivated sera in respect of amboceptor is rendered less 
The fact that a greater degree of complement fixation occurs 
2E 2 
evident. 
