550 Dr. E. W. A. Walker. [Feb. 18, 
inulin. On again growing them, first anaérobically (four weeks) and then 
aérobically (two weeks) in inulin medium, all regained the power of acting 
anaérobically on inulin, and all but G the power of acting on it in the 
presence of air. 
At intervals during the course of this experiment the organisms from 
inulin culture were sub-cultivated, and tested through the series of test 
media, with the results given in Table V below. They showed marked 
variations at different stages of the experiment. 
Table V.—Streptococci L, P, 8, G and M. Showing the Reactions of the 
Five Streptococci in the Test Media at Different Stages of the Inulin 
Experiment. 
g 
i 
g 3 a 
5 A : 43 mA ‘ ‘ ° 
8 Time of testing. ie S ‘ 2 eu 2 g om 
a a fe) a |. SI q aie 
a =| 82) (eos 
ro ° 
nD Slat | se he) elem 
L | 1. Original reactions, June, 1910 ............... OOo AE Ona Or se taeg a 
2. Atterio weeks 1m iaUlinyss. e865 meee seson Os ee OP ia Ona 
3. Atter Siweeks it imulin 1.42... eerste: +) +] +] +.) +] +] +78 
4. After 3 months in stab agar .................. OO ee) O04 soe OM aap 
5. After 2 weeks in inulin (anaérobic)......... +10] + |) 0 | 0) +) +422 
Os Avrendrot Cxperimenthp nes. 6. 10. eeeenn: 0; 0);,+ ] 0) +) + | OQ] 8 fds 
P | 1. Original reactions, June, 1910 ............... OF) 0.4) se ON Os a ae. 
2. After’ weeks in imtiliti:.«.:ssss.c-ce eee O 0 + | O + | + | 0 1 
JeAdter S/aveeks Inginwlain.ss 04s eeeeeeee OF Oo OE Ea OM mee 
4, After 3 months in stab agar ..............6... Of Oop OO el Or ae 
5. After 2 weeks in inulin (anaérobic)......... el On| OO ee al eee ae 
6. At end’of experiments, os. eee ee +/0O0}]+/]0]+,+ /].0 | 2) 6 
S |1. Original reactions, June, 1910 ............... Ole | lio avout 
2. -Aiter’> weeks in imulin, Ss csse eaten eee OO} +) 40) see ea Oe 
5. -Atters weeks inunulin sn.e ane ree tit f+ | tye 4 ae 
4. After 3 months in stab agar .................. OM) 0 4) +4) O01 SOR Ca) Cm ie 
5. After 2 weeks in inulin (anaérobic)......... lO. lb oO 1 OM ee Ost eel 
6. “At end of experiment: sce. cs3. sae een ece se +/ +/+} +}] +] +] 07)] 8 | 18 
G | 1. Original reactions, June, 1910 ............... +/+ ]},+;)+]0;]+4+ 1) 0 
2. After-o weeksiin inulini). sy jenn eee eee Oar | te OO ce a Osa aaa 
3. Adter8 weeks in analim cea: ceeeeee eee +/+] + ]+]+;)+]+] 8 
4, After 3 months in stab agar ..............00+- Ot ee [Oo Or | sO eee 
5. After 2 weeks in inulin (anaérobic)......... Oe) 4) OLS Oik) ess etn ee 
G. AG end: of experiment 1.5 ee eee eee cer i eaeai ce 4 | te 
M | 1. Original reactions, June, 1910 An Seine Oe Oe OT aio 
2) Atter’2 weeks anianwlinyace. eee eee ae Oe) bk a ee ees 
3. After 3 months in stab agar ...............+6 O;+i]+]+]+]+ ]+]0 
4, After 2 weeks in inulin (anaérobic)......... O} +] + |] + | 4) eee 
5. Abend of experiment... )--e. neers eee O;+]+i]+i]+]+i]+;]07; 8 
* After another week of cultivation in inulin medium this reaction became positive. 
