552 , Dr. E. W. A. Walker. *\ [Fabs mae 
aerobically, sub-cultures being made every three days directly from the 
mannite cultures to fresh mannite media. 
On December 5 all were still negative to mannite. The organisms. 
were now tested through the other test media, with the results shown above 
in Table VI. 
Milk Test. 
The streptococci L, P, 8S, G, M, were grown continuously in milk for well 
over four months, from June 17 until October 31, fresh subcultures being 
made in litmus milk once a week. 
When the experiment was begun only G clotted milk (on the second day). 
On July 15 S and P had begun to clot milk, while G now only clotted 
the milk on the fourth day of growth. 
On July 21 G had ceased to clot milk at all, and had not regained this. 
power by October 31, when the experiment was brought to an end. 
On August 19 S and P no longer clotted milk. On September 208 gave: 
a feeble clot. On October 2 L and P clotted the milk on the sixth day. On 
October 19 L, P, and S clotted the milk firmly in three days, and this. 
condition still held on October 31. M never clotted milk throughout the 
experiment. 
Thus, of the five streptococci, one lost the power of clotting milk, three 
gained it, and one remained unchanged. These five organisms were now put. 
Table VII.—Streptococci L, P, 8, G and M. Showing the Changes of 
Reactions which had taken place after Four Months’ Cultivation in 
Litmus Milk. 
a 
5 : o 
° 2 nD 
8 | Time of testing. g 5 2 2 & d = & 
= | ee ts le es eie es) || ce e 5 
| = 3 a g 5 | 3 SPU) 
72) | = DM eS an a 02) = @) 
i |/.dbstuns, 19107 te, ee | oO Ot 0 0 + 0 
| 2. After 4 months in milk; + 0 + = + “- O 3 
deg Ie dune; TOhOM ee ae ee 8) 0 + 0 0 + 0 
| 2. After 4 months in milk! » + 0 + O + + 0 2 
S 1. Jane, 1910) ee 0 Se ee 0 0 # 0 
| 2. After 4 months in milk, + 0 + + 8) 0 0 4, 
Ge ya ee dame, 910 Cee eee + + + + O + 8) 
2. ee 4, months in milk 0 + + + 0 + (0) il 
M be oune; TOTO accra ee 0 + + 0 0 + 0 
2. After 4 months in milk} 0 + |. + O O + | + 1 
