96 Mr. Plimmer. Effects on Rats of the Trypanosomata [Nov. 21, 



somata were found both in the centrifugalised blood and in smears made 

 from the spinal cord. The third rat (No. 68, of the 42nd inoculation) was 

 inoculated on September 26, 1905, and became paraplegic on December 13. 

 No trypanosomata could be found in ordinary blood films in 17 examinations 

 made during the disease, nor at the time of death. The animal died on 

 January 3, 1906, when a few trypanosomata were found in the centri- 

 fugalised blood, and in smears from the spinal cord. The fourth and fifth 

 rats (Nos. 101 and 102, of the 70th inoculation) were inoculated on May 26, 

 1906, and on July 17 No. 101 became paralysed in the right hind leg, and 

 on July 18 No. 102 also became paralysed in the right hind leg. Both of 

 these rats had a good number of trypanosomata in their blood of the ordinary 

 long, very motile kind. On July 19 No. 101 became completely paraplegic, 

 and died on the 22nd. No. 102 died on July 23, without the other leg 

 becoming paralysed. 



The following table gives a comparative outline of these five experi- 

 ments : — 



Eat. 



Duration of disease. 



Appearance of 

 paralytic symptoms. 



Trypanosomata in blood during 

 life. 



35 



42 



" 68 

 101 

 102 



41 days 



14th day 



Yery scanty. 



Not found ; few found post mortem 



in centrifugalised blood. 

 Ditto. 



Good number. 

 Ditto. 



64 days, rat killed... 

 102 days 



63rd day 



78th day 















It will be noted that, in Eats Nos. 101 and 102, trypanosomata were 

 present in good numbers in the blood, but in those cases (Nos. 42 and 68) 

 in which the paralysis was most marked, and which, to all appearances, were 

 exactly like rats paralysed after inoculation with the Sleeping Sickness strain 

 of trypanosomata, they were absent, or at any rate so few in number that 

 they could only be found in centrifugalised blood after death. From all of 

 these rats inoculations were made, with the constant result that the disease 

 in the inoculated animals reverted to the generalised form mentioned above 

 as characteristic of this strain of Gambia Fever. 



This strain of trypanosomata was also inoculated into a monkey, which 

 lived 33 days after inoculation, this being three days longer than the 

 average time of the 14 monkeys I have inoculated with the Sleeping Sickness 

 strain. Trypanosomata appeared in the blood 10 days after inoculation, and 

 were of the type of the Sleeping Sickness strain. Eats inoculated from this 

 monkey did not become paralysed ; trypanosomata of the Gambia Fever 



