1906.] of Gambia Fever and of Sleeping Sickness. 



97 



type were plentiful in their blood, and the disease ran the course of that 

 produced by this strain. 



2. Sleeping Sickness. 



In all, 82 rats have been inoculated with this strain of trypanosomata, in 

 59 direct series of inoculations, and, of these, 22 have been paralysed. 

 In these cases trypanosomata could be found only at intervals and rarely, 

 and sometimes not at all, in ordinary blood films, and in some cases none 

 could be found in the centrifugalised blood post mortem ; but in cases where 

 prolonged search was made a few were always found. The paralysis occurred 

 most generally in those rats which were inoculated direct from monkeys, and 

 it occurred less and less frequently as the passages got more remote, firstly from 

 the monkeys and secondly from the original monkey. I have succeeded 

 in producing paralysis once as far as the fourth passage from the monkey ; 

 generally after the second, in five instances after the first, passage the 

 trypanosomata have become generalised in the blood, and the symptoms and 

 course of the disease have been those of the Gambia Fever strain. 



The following table gives an outline of the instance mentioned above, in 

 which paralysis occurred in four successive passages : — 



Bats. 



Inoculated from 



Duration 



of 



disease. 



Appearance of 



paralysis after 



inoculation. 



Trypanosomata in 

 blood. 



Spleen. 



la 



Monkey which was 

 inoculated from the 

 monkey brought 

 home by Col. Bruce 



Eat 7a 



Rat8 



Rat 10a 



Eat 136 



mths. days. 

 P6 7 



14 

 



23 

 6 

 3 

 



15 



mths. days. 



5 15 



2 2 



5 2 



3 1 



3 



Not paralysed 



1 20 



Not paralysed 



None seen 



None seen 



None seen 



Few 



Few 



Few in every field 



Few 



Plentiful 



Not enlarged. 



Not enlarged. 



Not enlarged. 



Enlarged. 



Enlarged. 



Large. 



Large. 



Very large. 



The rats Eos. la and 7b at the head of the above series were inoculated from 

 a monkey which had been inoculated from the monkey Colonel Bruce, F.K.S., 

 brought home from Uganda, this monkey having been inoculated on the spot 

 with cerebro-spinal fluid from a case of advanced Sleeping Sickness. 



In rats inoculated from the 10th monkey done in direct succession from 

 Colonel Bruce's monkey, the paralysis occurred only in those inoculated 

 directly from the monkey, the next passage producing trypanosomata and 

 symptoms of the Gambia Fever type, as set forth in the following 

 table : — 



