98 Mr. Plimmer. Effects on Rats of the Trypanosomata [Nov. 21, 



Rat. 



Inoculated from 



Duration of 

 disease. 



Appearance of 



paralysis after 



inoculation. 



Try pano somata 

 in blood. 



Spleen. 



66a} 



66b f 

 66c J 

 68 



69 



70 



71 



Tenth monkey in f 

 direct successionl 

 from Col. Bruce's ] 

 monkey [, 



Rat 66a 



Rat 66b 



Rat 66c 



Rat 69 



Killed at 



3 mths. 25 days 



4 mths. 2 days 

 4 mths. 6 days 

 2 mths. 26 days 



2 mths. 1 day 

 Killed at 



1 mth. 20 days 



2 mths.' 18 days 



2 mths. 22 days 



3 mths. 11 days 

 3 mths. 22 days 



None 



None 

 None 



None 



None seen 



None seen 

 None seen 

 Few from 15th 

 day after inocu- 

 lation, increasing 

 till death 

 Plentiful 

 Fair number 



Plentiful 



Not enlarged. 



Not enlarged. 

 Not enlarged. 

 Large. 



Very large. 

 Large. 



Very large. 



3. Paralysis after Inoculation vAth Trypanosomata. 



With regard to the question of paralysis occurring in the rat after 

 inoculation with the above-mentioned particular strains of trypanosomata, it 

 does not appear to have come within the experience of all those who have , 

 worked with these particular organisms. For instance, neither Dr. Thomas, 

 of the Liverpool School of Tropical Medicine, nor Dr. Laveran, of Paris, 

 appear to have encountered it in their work with these trypanosomata. 



But Lieutenants Tulloch and Gray, in Uganda, have made a number of 

 experiments to test the statement made in my Preliminary Note, and have 

 recorded the same results in a paper published in No. VIII of the Sleeping 

 Sickness Reports of the Royal Society. 



In four rats inoculated with the blood of a monkey which had been 

 previously inoculated with cerebro-spinal fluid from Sleeping Sickness 

 patients, paralysis of the hind limbs occurred 10 or more days before death ; 

 and in three out of the four rats no trypanosomata could be found in the 

 peripheral blood at the time of death. They also found that rats did not show 

 any signs of infection after inoculation with the blood of Sleeping Sickness 

 patients, which has also been my experience. I inoculated two rats with 

 blood from a case of Sleeping Sickness, in University College Hospital, a year 

 before death, and two more shortly before death, on both of which occasions 

 trypanosomata were present in the patients' blood ; the results in both series 

 were negative. 



The site of the inoculation apparently determines the locality of the 

 paralysis. In all my experiments, with the exception of four, inoculation 

 was made at the inner side of the hind leg, and the paralysis occurred in the 

 lower limbs. Only in one rat, which lived nearly 11 months, did any weak- 

 ness of the forelegs show itself ; and this was due to extensive degeneration 



