1907.] 



Studies on Enzyme Action, 



353 



complete hydrolysis ; as the total change exceeded 200' in every case, the 

 error in the polarimetric reading being not more than about 1/, the values 

 found may be regarded as accurate to within about J per cent. 



Table I. — Action of Extracts of Top Yeast (S. Cerevisice) on Solutions of 

 Equimolecular Concentration at 25° and 35°. 



G-lucoside used. 



Yeast 

 extracted at 



Hydrolysis 

 effected at 



Amount hydrolysed in 



24 hours. 



48 hours. 



72 hours. 



Amygdalin < 



Maltose < 



Methyl- a-glucoside < 



25° 

 35 

 25 

 35 



25 

 35 

 25 

 35 



25 

 35 

 25 

 35 



25° 

 25 

 35 

 35 



25 

 25 

 35 

 35 



25 

 25 

 35 

 35 



per cent. 

 15-4 

 15-4 

 18*3 

 18-7 



72 3 

 21-8 

 65-5 

 11-7 



77-7 

 45-3 

 72-8 

 42-9 



per cent. 

 23-3 

 21-2 

 32-5 

 25-4 



93-8 

 26-9 

 72-6 

 15-9 



100-0 

 60-5 

 86-8 

 44-3 



per cent. 

 29-6 

 28-3 

 50-8 

 31-1 



100-0 

 30-3 

 69-9 



Table IT. — Action of Extracts of Top Yeast prepared at Temperatures 

 between 0° and 55° on Solutions of Equimolecular Concentrations. 









Amount hydrolysed in 



G-lucoside used. 



Yeast extracted at 



Hydrolysis 

 effected at 





















24 hours. 



48 hours. 



72 hours. 









per cent. 



per cent. 



per cent. 



r 



0° 



25° 



4-8 



13-4 



17-4 



i 



15 



15 



11-4 



16-4 



24-4 



1 



15 



25 



16-9 



24-5 



38-0 



Amygdalin ■{ 



15° (extract heated 

 at 45°— 1 hour) 



25 



15-1 



21-0 







45 



25 



16-1 



26-7 



36 



V 



55 



25 



racemised 



racemised 



racemised 



c 







25 



54-1 



69-6 



75-9 





15 



15 



57-9 



68-0 







15 



25 



70-0 



78-6 



80-4 



Maltose < 



15° (extract heated 

 at 45°— 1 hour) 



25 



3-9 



29-7 



32-3 



j 



45 



25 



1-8 



2-7 





I 



55 



25 



o-o 



o-o 



o-o 



r 







25 



49-0 



65-6 



74-9 





15 



15 



50-6 



64-9 





Methyl-o-glucoside \ 



15° (extract heated 

 at 45° — 1 hour) 



25 



39-4 



50'6 



63-9 



{ 



55 



25 



o-o 



o-o 



o-o 



