1907.] 



Studies on Enzyme Action. 



359 



Table VIII. — Action of Amygdalase from Top Yeast on Amygdalin in 

 presence of Maltose, Methyl-a-Glucoside, Glucose and Galactose at 25°. 

 The extract was prepared at 15° and subsequently heated at 50° during 

 two hours to kill the maltase. 



Substance hydrolysed. 



Rotatory power change at 25°. 



24 hours. 



48 hours. 72 hours. 



Amygdalin alone 



-0° 37' 

 + 0° 1' 



-0° 58' 

 + 0° 0' 



-1° 10' 



+ 0° 0' 



Maltose alone 



Sum 



-0° 36' 

 -0° 39' 



-0° 58' 

 -1° 0' 



-1° 10' 

 -1° 14' 





Retardation 



0° 3' 



0° 2' 



0° 4' 





Amy gdalin alone 



-0° 37' 

 + 0° 25' 



-0 C 58' 

 + 0° 42' 



-1° 10' 



+ 0° 54' 







-0° 12' 

 -0° 18' 



-0° 16' 



-0° 28' 



-0° 16' 



-0° 40' 



Amygdalin and methyl-a-glucoside together 

 Retardation 



0° 6' 



0° 12' 



0° 24' 



Amount hyd 



rolysed at 25° 



per cent. 

 17*5 

 10-4 

 16-7 



per cent. 

 26-2 

 24-1 

 28-3 











