418 Mr. W. B. Hardy. [Apr. 9, 



And this would account for the fact that the acid can be turned out of the 

 combination with globulin by an excess of neutral salt, but the alkali 

 cannot be. 



It is easy to prove by the boundary method that the globulin, when 

 dissolved by salts, takes no part in the electric transport. Dissociation of the 

 salt-globulin compound by the water may be regarded as being practically 

 completely suppressed by the excess salt present. This being the case, the 

 diminution of the electric conductivity of the salt by the globulin may be 

 used as a measure of the fraction of the salt actually combined with it. 



In the following table a few values are given. Comparison is made 

 between solutions containing the same amount of salt per litre ; but in the 

 one case the solution is saturated with globulin, in the other case it is a 

 simple solution of the salt in water. No correction is made for the diminu- 

 tion of the molecular conductivity of the salt owing to replacement of a 

 portion of the water by globulin, since it would amount to less than 0*01 per 

 -cent. No. Ill is of the nature of a control, to prove that electrolytic 

 impurities adherent to the purified globulin may be neglected. The salt 

 solution was made up with a dialysate in equilibrium with the suspension of 

 globulin. 



-Serum centrifuged, diluted to 10 vols., globulin precipitated by HA. The precipitate 

 suspended in a large volume of distilled water and collected by the centrifuge, and 

 re-suspended in water freed from gases by boiling. 



Specific conductivity of the water employed, 6 x 10 -6 rec. ohm, 18°. 



I. Ox globulin suspended in water. 100 c.c. = 4*08 grammes dry globulin. Tem- 

 perature, 18° : — 



Specific Specific 



conductivity, conductivity, 



salt globulin, salt water, K salt globulin 



Salt. Normality. x 10 5 . x 10 5 . K salt water ' 



X 2 S0 4 0-1716 1445 1539 0'938 



„ 0-1679 1411 1506 0-937 



NaCl 0-27 1850 1950 0-949 



Specific resistance of the globulin suspension 3'1 x 10 " 5 . 



II. Sheep globulin. 100 c.c. = 4*4 grammes : — 



NaCl 1 A OAKQ 2145 2280 °' 938 



„ J°* 2659 2150 2278 0-943 



The measurements in II were duplicated in order to test the degree of accuracy of the measure- 

 ments of volume. 



III. Ox globulin dialysed until in equilibrium with water saturated with toluol : — 



MgS0 4 0-11 517 543* 95 



In this case the salt solution ( # ) was made up with the dialysate, not with water. 



[Nate. — In a recent paper* it is stated that globulin in solution does not alter the 



* Mellanby, ' Journ. of Physiology,' vol. 33, p. 354, 1905. 



