Inhibitory Action upon Phagocytosis, etc. 483 



The difference between " T " and " C " was sufficiently striking, but it seemed desirable 

 to re-test the two fluids with stronger emulsions of B. tuberculosis and staphylococcus. 

 Accordingly, after standing in the cold room over-night at a temperature of 2° C, they 

 were again compared, with the following result : — 



Bacilli per leucocyte. 



Test fluid " T " + leucocytes + B. tuberculosis 0'8 



Control fluid " C " + leucocytes + B. tuberculosis 4'5 



Cocci per leucocyte. 



Test fluid " T " + leucocytes + staphylococcus 3*9 



Control fluid " C " + leucocytes + staphylococcus 24*5 



Thus the ratio of " C " to " T " in the case of tubercle rose after 16 hrs. from 4'6 : 1 to 

 5*6 : 1, and in the case of staphylococcus from 2*5 : 1 to 6*2 : 1. 



Experiment II. 



Fluids " T " and '* C " were prepared in the way described above and their opsonic 

 contents compared. Result : — 



Bacilli per leucocyte. 



Test fluid "T" 2'6 



Control fluid "C" 6'0 



Cocci per leucocyte. 



Test fluid "T" 4*2 



Control fluid "C" 17*2 



After standing over-night in the cold room, they were re-tested with fresh emulsions of 

 bacilli and cocci. Result : — 



Bacilli per leucocyte. 



Test fluid "T" TO 



Control fluid " C " 7'7 



Cocci per leucocyte. 



Test fluid "T" 2*3 



Control fluid " C " 9*0 



The ratio of "C" to "T" thus rose after 16 hrs. from 2*3 : 1 to 7*7 : 1 in the case of 

 B, tuberculosis, while in the case of staphylococcus it remained practically the same. 



EXPERCMENT III. 



In this experiment the heated serum and the salt solution were emulsified directly with 

 B. tuberculosis in an agate mortar. Result : — 



Bacilli per leucocyte. 



Test fluid "T" 1*2 



Control fluid "C" 5'0 



Cocci per leucocyte. 



Test fluid "T" 5*4 



Control fluid "C" 15*9 



Experiment IY. 



Instead of B. tuberculosis, staphylococci were brought in contact with heated normal 

 serum. The further steps in the preparation of " T " and " C " remained the same. 

 Result : — 



