568 Messrs. G. A. Buckmaster and J. A. Gardner. [Mar. 12, 



The general plan of the experiment was to anaesthetise the animal with 

 ether or nitrous oxide, allow the anaesthetic to be disengaged from the body, 

 and then administer a known percentage of chloroform. The determination 

 of the chloroform-content of the blood was made at the asphyxial point. 

 The animal was then allowed to recover from the full effect of the 

 chloroform, either naturally or by artificial respiration, and sufficient time 

 was allowed for practically all the chloroform to be eliminated from the 

 blood. One hour to one and a-half hours was usually sufficient for this, and 

 it was generally necessary to give the animal a little ether or nitrous oxide in 

 order to preserve unconsciousness. During this period the animal was bled 

 from the carotid artery to a known amount. The quantity of blood 

 withdrawn varied with the weight of the animal. Sometimes the whole 

 amount was taken at once, but generally in successive small quantities. The 

 animal was re-anaesthetised and another determination of the amount of 

 chloroform in the blood made at the asphyxial point, but before re-anaes- 

 thetisation a sample of blood was always taken as a control. The details of 

 the experiments are recorded in Table I. 



These are the only experiments in which the respiration-curves definitely 

 indicated that asphyxia occurred both before and after bleeding in the 

 initial stage of anaesthesia. In five of the seven experiments, comparative 

 determinations were made before and after bleeding. Of these, in two cases 

 the percentages of chloroform found in the blood were practically identical. 

 In two cases the percentage was higher after than before bleeding, and in 

 one case lower. In the other two experiments in which values after bleeding 

 were obtained, the figures are fairly normal. 



Before bleeding the average percentage of chloroform in 100 grammes of 

 blood was approximately 0'043 gramme and after bleeding 0*045 gramme. 

 It would appear, therefore, that in the above experiments the percentage of 

 chloroform in the blood is not altered by bleeding. 



Experiments in whicli Asphyxia took place during the Second. Stage of 



Anaesthesia. 

 In these experiments chloroform was inhaled from the Woulff's bottle 

 described at length in a former paper " On the Eate of the Assumption of 

 Chloroform by the Blood during Anaesthesia." In those cases where 

 respiration stopped within the first few minutes, the animal was brought 

 round artificially or allowed to recover naturally and the anaesthetic again 

 administered continuously. In other respects the procedure was the same 

 as in the first group of experiments. The details of the experiments are 

 recorded in Table II. 



