572 Messrs. G. A. Buckmaster and J. A. Gardner. [Mar. 12, 



It will be seen, therefore, that the average percentage of chloroform in the 

 blood at the asphyxial point is 0*048 before and 0*051 after bleeding, or 

 eliminating the first two figures in Column 9, quoted in Experiment XIII, and 

 the first figure in same column in Experiment XIV, as these values were 

 determined in samples taken considerably before asphyxia, the corrected 

 averages are 005 before and 0*0497 after bleeding. In one experiment the 

 figures were sensibly the same before and after withdrawal of blood, and in four 

 experiments there was a slight increase after the withdrawal of blood. In 

 the two remaining experiments a decrease was observed after a haemorrhage. 



In the following table (No. Ill), we quote two experiments in which the 

 respiration tracings showed that asphyxia took place before bleeding in the 

 second stage of anaesthesia, and after bleeding at the initial stage. 



Experiments in which comparisons were made of the percentage of chloroform 

 in the Hood at the asphyxial stage after hemorrhage and after the replacement 

 of the blood which had been removed by the blood of another animal of the same 

 species. All the observations were made in the second stage of anaesthesia. 



The replacement of blood was effected in the following way : — A cat was 

 anassthetised with ether and a cannula placed in the carotid artery. The 

 cannula was connected by a short rubber tube fitted with a clip, with the 

 outflow end of a burette. This was warmed and smeared inside with olive 

 oil, and before making connection with the cannula, the cannula, rubber tube 

 and about an inch of the burette were filled with warm olive oil, so that the 

 blood could be received from the animal without coming in contact with the 

 air or glass, and without cooling to any serious extent. A small cannula had 

 been previously placed in the femoral vein of the animal which was the 

 subject of the anaesthetic experiment. This cannula was filled with a warm 

 solution of sodium sulphate of a strength isotonic with that of blood plasma. 

 The burette was now disconnected from the cannula in the carotid artery, and 

 connected with the cannula in the femoral vein, care being takeu that no air 

 entered the circulation. The requisite amount of blood was then allowed 

 slowly to flow during the course of two or three minutes into the animaL 

 The whole transfer of blood was effected in less than four minutes. In none 

 of our experiments did we notice any disturbing effects produced by the 

 introduction of the blood. The animal was then re-anaesthetised with 

 chloroform, after recovering from the first anaesthetic, precisely as in the first 

 part of the experiment. Details of these experiments are recorded in 

 Table IV. 



In two out of the three complete experiments the percentage of chloroform 

 in the blood at the asphyxial point after bleeding and after replacement 



