1890.] occurring in the Neighbourhood of Simla. 87 



The teleutospores are brilliant orange yellow in colour, and are 

 usually single celled, and somewhat thickened at the free end, and the 

 whole bed is covered externally with a thin hyaline layer (fig. 5, PL II). 

 Each spore measures about 13/i in breadth, and 30 to 35/* in length : 

 each usually displays a clear nucleolar space. When a portion of leaf 

 blade containing teleutospore beds is kept in a moist atmosphere the 

 latter are found after some hours covered with minute orange red hairs, 

 just distinguishable with the naked eye. These are the promycelial 

 tubes which each bear a very large oval deeply orange red sporidium, 

 measuring about 27 by 15/*, attached asymetrically to the sterigmata. 

 These sporidia germinate readily in water throwing out a simple germ 

 tube, into which the orange red contents wander. As affected bushes 

 have usually immense numbers of pustules on almost every leaf I 

 thought there might be a perennial mycelium • but an examination of 

 the stem bearing numerous such leaves showed no trace of mycelium. 



A very remarkable peculiarity in this fungus is the occurrence of 

 hypertrophies on the leaves and smaller stems, bearing Puccinia pustules. 

 It is so extraordinary that one is inclined to believe that it is an acci- 

 dental association of two parasitic fungi, each perfectly independent ; 

 and this view commends itself the more favourably when I note that 

 I never found these Puccinia hypertrophies on any other than one parti- 

 cular bush. On this bush, however, I collected many, and a few of them 

 were on leaves bearing immense numbers of Goleosporium teleutospores. 

 As the Puccinia were so intimately associated with the Goleosporium I 

 will note its chai'acters here, leaving the final determination of accidental 

 association, or relationship, to future biological experiment. 



I found these Puccinia hypertrophies on the 7th August, when the 

 Coleosporium is in full growth, on the stem, petiole, and leaf blades. 

 The hypertrophies were studded with black pustules containing Puccinia 

 spores. The spores are firmly adherent, and when scraped off appear 

 brownish yellow to the naked eye. By transmitted light they are pale 

 brownish yellow bodies, with thin walls, and very clearly defined nucleo- 

 lar spaces in each cell. They are clearly, though not deeply, constricted 

 at the septum ; sometimes with a slight apical thickening, but oftener not. 

 Externally they are smooth (fig. 7, PI. II). The fresh spores examined 

 in water measured from 42 to 47/* in total length, and 20 to 24/* in 

 breadth at the septum, which divides the spore into two almost equal 

 halves. The stalks adhering to the scraped off spores are very long, 

 measuring in diameter 5/* at the far end to 10/* at the insertion into the 

 spore. I placed these spores into water with a view to observing their 

 germination : but they do not germinate apparently until after a period 

 of rest. 



