
Influence of Cobra-venom on the Proteid Metabolism. 167 

enumerated, duplicate analyses were made in all cases and the mean of these 
taken :-— 
1. Total nitrogen by Argutinsky’s modification of Kjeldahl’s method. 
2. Nitrogen in urea by Bohland’s method by precipitating with phospho- 
tungstic (Merck’s) and hydrochloric acids. 
3. Nitrogen of ammonia by Schlossing’s method. 
4. Nitrogen not in urea (non-urea nitrogen) was calculated by difference. 
5. Nitrogen in purin bodies by Kriiger and Wulff’s method.* 
6. Phosphoric acid as P,O; by titrating with uranium nitrate. 
7. The percentages of nitrogen in urea, not in urea (non-urea nitrogen), in 
ammonia, in purin bodies, in other compounds were calculated in terms of 
total nitrogen. 
8. The percentages of P,O, were calculated in terms of total nitrogen. 
Averages of the figures before and after the injections were determined. 
Albumin was tested for by cold nitric acid and by heat and acetic acid. 
Some of the experiments were rendered valueless by the early development 
of an abscess at the seat of inoculation, but the following were carried on, 
without disturbance from such accidents. 
In all the experiments no marked symptoms were produced. In some 
cases after injection the dog lay very quiet for a few hours. In a few there 
was a local swelling observed next day, but it soon disappeared. 
Hapervment 1. 
For this experiment a retriever weighing 18 kilogrammes was used. The dog 
was kept in its cage for two or three days before the examination of the urine 
was begun, so that it might get accustomed to its diet and nitrogenous 
equilibrium be established. 
Food Analyses. 
Analyses of the food given to the dog showed the amount of nitrogen to 
be :— | 

Waly diet: 500 ec. milk.....5.0.........000. = 1:83 grammes nitrogen 
200 grammes oatmeal ......... = 4:30 3 
Total intake of nitrogen per diem... = 613 x 
Analyses of the urine were begun on November 16, 1903, and on the 19th, 
at 4 P.M. 1:25 milligrammes Cobra-venom dissolved in 0°5 cc. sterilised 
normal saline solution was injected subcutaneously in the side. 
* Cf. ‘Zeit. fiir Phys. Chem.,’ vol. 20, p. 177, 1895. 
