
172 Dr. J. Scott. The Influence of [Feb. 6, 
Table 
Nitrogen. 
| Total Nitrogen Nitrogen 
Quantity Re- nitrogen. as urea. not as urea. 
Date. of urine | Sp. gr. ou ae 
in ¢.c. eee 
Per- Per- Per- 
centage Per | centage Per | centage Per 
of diem. of diem. of diem. 
urine. urine. urine. 
20 milligrammes Cobra venom (two minimum 






1904— 
PAD ETS ache cea No urine | passed. 
f 760 
Be ean: { to fro acid | 0°603 6°03 | 0°518 5°18 | 0:085 0°85 
Lj; 1000 
690 
FF aod (U soncernane / to ; 1015 acid | 0°483 4°83 | 0°409 4:09 | 0:074 0 ‘74 
1000 
500 
Afaaie aren: ; to f 1015 acid | 0°440 4°40 | 0:°372 3°72 | 0-068 0°68 
1000 
Averages after 

PAVIA EO MeL OTe Ls ss sists Badin tye exonerate rece | 0 °509 | 5 ‘09 | 0 *433 | 4°33 | 0 ‘076 | 0°76 

The minimum lethal dose of the venom as determined is 0:00025 gramme per 
kilogramme of body weight. Aseptic precautions were used. The effects of this 
injection were studied for five days and a second injection of 2°5 milligrammes 
Cobra venom dissolved in 1 c.c. saline solution was injected on the 25th at 
9.30 A.M. and the examination of the urine carried on till November 30. 
Hapervment 2. 
For this experiment a collie, weighing 17 kilogrammes, was used. The 
same precautions were taken and the same amount of food given. 
The analyses of the urine were begun on March 22, 1904, and on the 26th 
5 milligrammes Cobra venom in 0°5 c.e. sterilised normal saline solution were 
injected subcutaneously in the side at 11 a.m. A few hours after the injection 
the dog vomited, but there was no other symptom. The effects of the venom 
were studied till the 30th. 
_— 
