190 Mr. J. A. Craw. On the Physical [Mar, 22, 
an apparently true equilibrium results. From Table V the irreversibility 
in nearly neutral solutions gives a slight effect in the opposite direction to the 
effect obtained by Danysz and von Dungern. 
On the Mass Action of Lysin and Antilysin. 
If the equation, toxin+antitoxin = 2 (toxin, antitoxin), proposed by 
Arrhenius and Madsen, holds for lysin and antilysin, then, when the same 
relative concentrations of these bodies are brought together, the equilibria 
attained will be the same, 2.e., the relative concentrations of the substances 
will be independent of the volume of the mixture. Thus, 10 cc. of a 
mixture of lysin and antilysin in equilibrium will contain as much free lysin 
as 1 cc. of a mixture 10 times as strong in total lysin and antilysin. — 
In Table VI (a 1, 2) and (a 5, 6) are such mixtures which were brought to 
equilibrium at 37°, the test volume (3) of the first mixture was 1 c.c., and. 
of the other 0:1 c.c., added with 0°9 c.c. saline (7). The hemolytic indices 
(4) (8) are not equal, being respectively (50) and (60). 
Table V1.—Heemolytic Indices of Multiple Mixtures of Lysin and Antilysin 
in Equilibrium. 



1 ho 3, | 3 A | 5 | 6. | 7 | 8 
Lysin Antilysin | Test Stern Lysin | Antilysin | Test reas 
cone. cone. volume conc. cone. volume 
3 index. : index 
per c.c. perce. in ¢c.c. per ¢.c, || perc.e. jan’ Weve: 
1 EE rca 0 ‘005 0-01 1°0 50 0°05 O°l O°l 60 
an ena 0 :0025 0 :005 1:0 26 0-025 0°05 0:1 50 
Ca ee O 0005 0-001 1:0 O 0°05 O°'l 0:01 42 °5 
(i ae Ne 0 :00025 0 ‘0005 1:0 O 0 :025 0°05 0°01 Al 
Two similarly related mixtures of half the strength (61, 2) and (0 5, 6) 
showed a more marked difference, viz. (26) and (50). When the strength of 
the mixtures were widely different as 1—100, the difference is extremely 
marked, ¢.g., (¢ 1,2) and (¢5,6) gave the indices (0) and (42°5) when 1 ce. 
and 0:01 c.c. with 0°99 cc. saline were used as the test volume respectively. 
Two similarly related mixtures of half the strength (d 1, 2) and (d 9, 6) showed 
a similar effect and indices (0) and (41). 
It must then be concluded that Arrhenius and Madsen’s equation does 
not apply to the lysin-antilysin reaction so far as megatherium lysin is 
concerned. 
