240 Dr. F. W. Mott. Brains of Men and Anmmals [Mar. 14, 
chromatin bodies which are seen in the vessels. If these chromatin bodies, as 
Leishman would affirm, are the macro-nuclei of trypanosomes, then it is 
difficult to explain why a dozen or more of the chromatin bodies can some- 
times be seen lying in a space which would be covered by one trypanosome. 
Still the trypanosomes may have degenerated elsewhere, and the macro-nuclei 
be carried into the capillaries. In view, however, of the researches of 
Captain Rogers regarding Leishman bodies being altered phases of trypano- 
somes, and the contention of Plimmer and Bradford ve the existence of 
amoeboid forms of trypanosomes, it 1s possible that these chromatin bodies may 
be some phases in the life of the trypanosome in the blood; and it may be 
mentioned in support of this (although I do not profess to dispute the opinion 
of biologists who have studied the question) that after a very careful search 
of a large number of sections, I have been unable to see a single trypano- 
some or a degenerated one, which is quite different to what one found in 
the brain of the rabbit inoculated with Surra. 
Hapervment 202. Ox—This animal died within three months of infection, the 
same portions of brain were examined. It was only after some careful 
searching that I could find a few small vessels containing these chromatin 
bodies. A drawing is given to illustrate these vessels. It will be observed 
that there are a far larger number of minute, just visible, stained particles. 
Addendum.—Since reading this preliminary note, by a new method of 
staining I have found trypanosomes, and what I believe are Plimmer and 
Bradford’s amceboid forms in ox, Experiment 202. 
Clearly, then, at any rate in the brain, the evidence of the existence of 
trypanosomes in the blood of animals dying of trypanosome disease may vary 
very considerably. In view of the fact that blood in which no trypanosomes 
can be detected microscopically, yet by culture experiments they may be 
obtained, it may be asked (although here, again, I do not pose as an authority) 
whether these bodies with chromatin particles can develop into trypanosomes. 
In the vessels of the brain of the Ox 162, many leucocytes can be seen 
which have taken up the chromatin bodies. It may be mentioned that in 
these two cases there is no sign of meningo-encephalitis, and there was no 
diplo-streptococcal infection. The ganglion cells showed chromolytic changes, 
and there were many minute capillary hemorrhages, probably due to plugging 
of the capillaries by the organisms. 
Moreover, there were curious cells lying free in the vessels, which, how- 
ever, I could not assert were not detached endothelial cells with nuclear 
changes, except that I have not observed such appearances before. (Vide 
drawing.) 
