380 Major W. H. Horrocks. (Goats as a [ July, 
On June 22 the milk was again examined and the IM. melitensis recovered 
once more. 
On June 23 examination of the urine was commenced. The vagina was 
washed out with an antiseptic solution and a catheter, previously sterilised in 
boiling water, passed into the bladder. The urine so obtained was plated on 
litmus-nutrose-agar, but after four days’ incubation at 37° C., in spite of the 
precautions taken, the plates were found densely crowded with saprophytic 
organisms, and the M. melitensis could not be detected. 
On June 24 and 26 the urine was again plated, the same precautions being 
used, but the plates were densely crowded with foreign organisms, and the 
M. melitensis could not be seen. 
On June 27, 28, 29, and 30, and on July 1, 3, 4, 5, 7, 8, 9, and 10, the 
urine was also examined, but up to the present the J. melitensis has not been 
recovered. 
The milk was plated again in June and July, and the MM. melitensis was 
found on each occasion. 
feswlt.—The M. melitensis appears to be steadily excreted in the apparently 
normal milk of this goat, but up to the present it has not been found in the 
urine. 
Goat No. 1.—This animal appeared healthy, but the udders were flaccid, 
and the milk exuded had a thin serous appearance. The temperature was 
taken regularly, but no indications of fever were observed. 
On June 22, 1 cc. of the milk was centrifugalised and the deposit plated. 
After four days’ incubation at 37° C., the plates were found so densely 
crowded with colonies of the M. melitensis that an accurate count could not 
be made. 
On June 24 and 26 the milk was again examined and similar results were 
obtained. 
On June 26, 29, and 30 the urine was examined, but no signs of the 
M. melitensis could be discovered. 
On July 1, 10 cc. of the urine were centrifugalised and the deposit plated ; 
four days later every plate was found studded with colonies of the specific 
microbe. The colonies were fished, planted on agar slopes, and the resulting 
growths tested in the usual manner. 
Result—The M. melitensis is excreted in very large numbers in the serous 
looking milk of this goat. It is also excreted in the urine. 
Goat No. 2.—This goat appeared quite well, and the milk exuded from the 
udders had a normal appearance. There were no indications of fever. 
On June 22, 1 cc. was centrifugalised and the deposit plated. After four 
days’ incubation about 30 colonies appeared in every plate. On June 24 
