488 Prof. J. T. Cash and Dr. W. R. Dunstan. [May 24, 
52 hours.—Legs drawn up. Cannot get off dorsum after voluntary 
effort, ability for further movement is temporarily suspended. 
72 hours.—Lethargic, sitting up, hop short, slight tremor on movement. 
1‘7 per kilogramme.—As above. Circulation in 24 hours is only just 
moving in larger vessels of web. 
48 hours.—Circulation still feeble, but more general; when placed on 
dorsum, a very slight movement in trunk. 
96 hours.—Eye reflex doubtful, but brisk leg reflex when placed on 
dorsum. Circulation feeble. Respiration is nil. 
120 hours——Cannot get off dorsum, but moves spontaneously. Eye 
reflex present. Faint respiratory movements at long intervals. 
144 hours.—Cannot get off dorsum, but crawls if placed on belly. Respira- 
tion stronger and more frequent. 
168 hours.—Gets off back, still feeble, spring very short. 
Proportions above 1°7 were lethal from failure of the heart, and so on one 
occasion was 1°5. It has been impossible, from scarcity of material, to make 
this estimation more exactly, but it is probable that the toxicity of the two 
pseudaconines is identical (1°75 gramme per kilogramme), although the 
duration of action of large doses of the indaconitine product appears to be 
relatively somewhat longer, a result which may be attributable rather to 
variations in the animals than to differences in the substances. 
Perfusion of the Frog’s Heart.—Solutions of pseudaconine (from indaconi- 
tine) salt (0°01 in 20 c.c. of menstruum) were found to increase the strength 
of the systole, and otherwise to occasion the same phenomena as those 
described for pseudaconine from pseudaconitine. The effect of large doses of 
the former seemed slightly in excess of that of the latter, but from the nature 
of the experiment exact contrast is difficult. The excitability of the prepara- 
tion beating spontaneously so long as perfused by Ringer’s solution, seems to 
decline on substituting pseudaconine solution, spontaneous contraction tending 
to become less frequent or to disappear. A good contraction is, however, 
elicited on stimulating. 
Action on Muscle-nerve-—The same phenomena are occasioned by both 
pseudaconines. 
Experiment.—Pegged LF. temporaria, vascular ligature applied to left leg. 
Injection 0°3 per kilogramme pseudaconine (from indaconitine). In 20’, and 
before reflex was quite abolished, two companion nerve-muscle preparations 
were made. The effect of faradisation (Gi of nerve and Ge of muscle) is 
shown in the figures. Each stimulation is of 3’ duration, and it is 
observable that the longer the rest interval and the greater the ensuing 
