
1905.] Substance in Serum which influences Phagocytosis. 519 
Cocci counted in 
22 leucocytes. 
No. of ingested 
Horse 2—continued. Vol. cocci per leucocyte. 
Washed leucocytes of horse serum.................. L 
SET UMM OL HOTWAL HOTSE..-... 1c. scccnevececseceiceeseres 1 } 3 
+ Emulsion of streptococcus from agar ............ 1 
Horse 3— 
Washed leucocytes of horse serum ............... 1 
BRSCGUI OF EPOUSE Ossie c. oc. ccoesescccseccsececcesan cases if 23 
+ Emulsion of streptococcus from agar ............ 1 
Washed leucocytes of horse serum ............... 1 
+Serum of normal horse ...............ceceeeeeeseenee if 4 
+ Emulsion of streptococcus from agar ............ 1 
Typhoid Bacillus. 
Bacilli contained in 
22 polymorphonuclear 
leucocytes. No. of ingested 
Vol. bacilli per leucocyte. 
Serum of normal horse heated to 60° C. for 
PU MUMELURLES! 2). ices eiid’snne sence cenaweig vos os tudadaepiite 1 
+ Washed horse leucocytes ..............cecceeeeeeeoeees i} 22 
+ Emulsion of typhoid bacillus ....................008 1 
Serum of immune horse heated to 60° C. for 20 
HUMMUS ese Santos a veld sisesacsieceuasiisaseestieu esas ds cies 1 
+ Washed horse leucocytes ..............cceeseeeseeeeees | 49 
+ Emulsion of typhoid bacillus ...................ee0ee 1 
Dysentery Bacillus (Shiga). 
Serum of normal horse heated to 60° C. for 20 
MITATNUDU ES, Pee Sale ws hire tosses Sess aeciues shee come coadeemea tes 1 
FA Wastied horse leucocytes ...3....)...scesessecemes cece i} es: 
+ Emulsion of dysentery bacillus ..................... 1 
Serum of immune horse heated to 60° C. for 20 
PTUENUUE CH Mani asins 2 uc.c' te bclecceb eG naiccied Peunawitinnseds wwe ) 
+ Washed horse leucocytes ...............sceceeseesecees 1 [ 25 
+ Emulsion of dysentery bacillus ...................4- 1. 
In the case of the typhoid and dysentery bacilli considerable difficulty was 
found in estimating the phagocytosis on account of the agglutination. The 
leucocytes, crammed full of bacilli, were found in some cases lying in groups 
close to bacillary clumps, in other cases it seemed as if the leucocytes when 
full of microbes tended themselves to become agglutinated. 
It is possible that an agglutination of the bacilli towards the leucocytes 
may be a part of the process which enables the leucocyte by the movements 
of its own protoplasm to englobe the microbe. The extraordinary rapidity 
