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p- 358. 
The Phagocytosis of Red Blood-Cells. 
By J. O. WAKELIN Barratt, M.D., B.Sc. Lond., British Medical Association 
Research Student. 
(From the Hygienisches Institut, Munich. Communicated by Sir Victor Horsley, 
F.R.S. Received July 14, 1905.) 
It has been shown by Savtschenko,* that when an animal has received 
injections of red blood-cells, its serum (inactivated by heating) causes the 
appearance of phagocytosis 7m vitro when leucocytes and red blood-cells of 
the kind used for injection are added, and the whole maintained at the tem- 
perature of the body. This action is attributed by Savtschenko to the action 
on the red blood-cells of amboceptor (immunisine, fixateur) contained in the 
serum employed. 
In order to obtain further information respecting the factors which 
determine phagocytosis am vitro of red blood-cells, a comparative examina- 
tion of the action of sera of animals nearly related to, and widely separated 
from, those supplying the erythrocytes used for injection was undertaken. 
This investigation confirmed the above observation, that leucocytes placed in 
the inactivated serum of injected animals ingested red blood-cells of the 
kind used for injection. In the course of this investigation, however, it was 
found that phagocytosis could be brought about by the serum of the injected 
animals when the serum was free from amboceptor, for the red blood-cells 
injected, as the following experiment shows :— 
Lapervment 1.—Into the abdominal cavity of a dove, the red blood-cells 
* “Du Réle des Immunesines;(Fixateurs) dans la Phagocytose,” ‘ Annales de I’ Institut 
Pasteur,’ 1902, vol. 16, p. 106. 
