990 Dr. H. W. Thomas. The Haperimental [Apr. 8, 
(c) Atoxyl and trypan red combined. 
The arsenic preparation given first and then followed in 36 to 72 hours 
with high doses of the dye subcutaneously. 
(¢) Trypan red alone. 
All of these methods of treatment were continued over a period of 
several weeks to three months, or until a decided improvement in the 
general condition of the animal was noticed, especially increase of weight, 
rise in hemoglobin and number of erythrocytes, with absence from the 
blood of the parasite. 
In nearly all cases controls were used. In every case the treated animals 
have survived their controls. From time to time blood was taken from the 
treated animals and susceptible animals were inoculated. After a varying 
length of time treatment was discontinued, and some of these animals, after 
a period of one to three months without treatment, were bled to death, and 
the whole blood injected into healthy animals. Such control animals have 
remained uninfected. 
RESULTS. 
Treatments A and B. 
T. gambiense.—Rabbits, guinea-pigs, and rats after one and a-half to 
three months’ treatment have survived. Treatment dis- 
continued four to five months ago. 
T. evanst.—One rabbit, two guinea-pigs are alive three months after 
stopping treatment. It is now seven months since the 
guinea-pigs were infected. 
T. brucei.— Four guinea-pigs, three months after treatment, were bled, 
and rats inoculated with whole heart blood have remained 
uninfected during one and a-quarter months. Rabbit two 
months treated, one month later still well. Twenty rats 
have survived four months. One rat infected on fifth 
day of disease when parasites were present, 150 or more 
to a field, living, and blood negative 84 days later. 
T. equinum.—One rabbit, treatment begun only when characteristic 
discharge from eyes, nose, etc., appeared—treatment for 
two months—discontinued one month, animal apparently 
well. Two guinea-pigs have survived three months 
without treatment. Rats, so-far, 101 days. 
T. equiperdum.—Pups, one has died from over dose, other negative and 
general condition better. 
LT. dimorphon.—Results not so good. Animals have lived a far longer 
time than controls, but no apparent cure can be recorded. 
