1905. | Treatment of Trypanosomiasis in Animals. 591 
Treatment C. 
With this method animals require treatment for a shorter period, but 
many have died from toxic effects of the dye; this is especially the case 
with dogs, pups, and kittens. | 
-T. dimorphon.—Results of combined treatment far more favourable. 
Experiments in progress. Trypan red alone. 
Results with 7. equinwm, rats have lived 197 days. 
Effects on animals infected with 7. gambiense, JT. evansi, T. brucet, 
T. dimorphon, T. equiperdwm, are in accordance with Laveran’s experiments. 
No definite curative powers remarked. 
Experiments with the sodium arseniate treatment of animals infected with 
the various trypanosomes have given less favourable results. Greater 
tendency is noted for toxic symptoms and sloughing to occur. High doses 
cannot be tolerated for a prolonged period. Jn my hands the arsenic-aniline 
compound has given far better results than treatment with sodium arseniate. 
The advantages of its administration intravenously or subcutaneously in high 
doses over a length of tume, namely, its less toxic properties, the absence of all 
tendency to cause sloughing, and the apparently longer action of the drug, make 
me believe that the employment of this compound is indicated in the treatment of 
human trypanosomiasis. 
The combination with trypan red is worth trying in small doses per os, 
but the appearance of any indication of nephritis or other toxic symptoms 
should cause the treatment to be immediately stopped. I have administered 
in pill-form six to eight grains three times a week for a period of three and 
a-half weeks without any untoward symptoms. The case was a native 
suffering from trypanosomiasis ; the parasites lessened in number, and by the 
end of the third week were hardly ever seen. 
Ehrlich and Shiga found that they could protect animals if trypan red 
was administered (either subcutaneously or per os). After five to seven 
days such animals could be injected with virulent blood containing 
trypanosomes without infection occurring. 
I have injected a goat with increasing doses of trypan red, and, after a 
time, used its serum. Mice infected with the Mal de Caderas parasite were 
injected with small quantities of this serum. In four cases the animals 
lived 31 to 48 days. Experiments in progress. 
