Figure 1. --Fractionation of ethanol extract. 
(Weights not corrected for moisture in tobacco) 
Ethanol extract (11, 786 g.) 
Solubles (El) (8,500 g.) 
Centrifuge after 
further storage 
at.0°1C: 
Ppt. (E2) 
(37 g.) 
Add a hexane 
Upper layer (E5) 
(1, 587 g.) (6,876 g.) 
Concentrate, 
filter 
Insolubles Solubles 
(E6) (6 g.) 
Impregnate on 
celite, percolate 
with solvents 
Petroleum Ether Acetone Methanol 
ether (E7) (E8) (EQ) (E10) 
(613 g.) (429 g.) (4llg.) (128 g.) 
Decantate (E3) 
(8, 463 g.) 
Lower layer (E4) 
(OO. 
Insolubles (EP1) (3, 286 g.) 
Extract with 
solvents 
H20 (EP2) 90% MeOH Et20 Hexane 
(2,936 g.) ¢ | | 
Pool 
——— 
Neutrals (EP3) Acids and bases 
(140 g.) (EP4) (210 g.) 
Add water, filter 
Solubles (E12) 
(6, 719 g.) 
Ppt. (Ell) 
(157 g.) 
Extract with water 
Solubles (E13) 
(88.6 g.) 
Insolubles (E14) 
(68.5 g.) 
