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Wash. Symptoms and Effects of Fluoride Fumigation on Various 



Ornamental and Crop Plants . To learn (l) and record photo- 

 graphically the sensitivity of forest, ornamental, vegetable, 

 and agronomic plants to small quantities of atmospheric 

 fluoride; (2) effects of exposure-period on plant response 

 to small amounts of atmospheric fluoride; (3) rate and extent 

 of fluoride upbuild in plants exposed to fluoride-contaminated 

 air; (A) fluoride content of forest, horticultural, and 

 agronomic plants grown in Spokane area, and (5) by terminal 

 and/or radial growth studies if any common fruit or ornamental 

 tree species in Spokane area have been adversely affected 

 by fluoride from local industrial operations. 

 PI. Path. 1143 (W-39) 



Wash. Application of Lyophilization in the Preservation and 



Study of Plant Disease Organisms . Establish (l) means for 

 preserving plant pathogens for several years; (2) techniques 

 to enhance longevity of lyophilized cultures involving studies 

 with suspending media, conditions of growth, age of cultures 

 to be lyophilized, concentration of spores in given suspension, 

 degree of vacuum and drying temperatures, storage factors and 

 evaluation methods in regard to viability and virulence; (3) 

 standard procedures for embedding lyophilized, diseased tissues 

 for histological and microtechnical investigations that bypass 

 present methods; (4) ways to preserve fragile, perishable gross- 

 specimens by substituting plastics or other materials for water 

 removed from specimens through lyophilization. 

 PI. Path. 1285 



