170 Messrs. 8. G. Shattock and L. 8. Dudgeon. [Dee. 21, 
show that in immune serum the phagocytosis of melanin may be largely | 
increased, 7.¢., that melanin is capable of being opsonised. So, when opsonin 
is removed by heating the immune serum, the phagocytosis of normal cells is 
notably reduced. 
The indices, commencing with that showing the least reduction, run :— 
0°67, 0:23, 016, 0°16. 
Question 4.—Will an increased phagocytosis of melanin take place if 
melanin is presented to the washed leucocytes of an immune blood in normal 
serum, aS compared with normal washed leucocvtes in normal serum ? 
The cases selected comprised the following: acute erysipelas, chronic bone 
abscess, acute pneumonia, lymphangitis, streptococcal pyemia, acute cellulitis, 
acute peritonitis. Of some of these more than one example was used. 
Summary and Conclusions from the Observations made to test the Preceding 
Question. 
In the case where melanin is presented to normal washed cells and to the 
patient’s washed cells, both in normal serum, the phagocytosis is, in some, 
below par; in others, above ; in others, at par. 
The indices run :— 
0:46.00", 21, AGolie Sale ali oe Gs ela me pono 
Question 5.—Will the phagocytosis be the same if melanin is presented to 
the washed cells of the patient, and to washed normal cells, in immune serum ? 
In the following experiments, the action of normal cells and patient's cells 
was tested in the patient’s serum. 
CasE.— Streptococcus pyogenes pyzmia. 
Index :7.,0-8-; USF 
CasE.— Acute pneumonia ; 5th day of disease ; male, zt. 23 ; temperature 103° F. 
Index tres.c. 3°3. 
In each of these observations, the work done by the patient's cells is 
greater than that done by normal cells, the indices being 1:7, 3°3. 
In the four following observations, the phagocytosis of leucocytes from 
different cases of Bacillus coli infection, in the same immune serum is set 
forth, as compared with that of normal cells in the same immune serum. 
The immune serum was from a ease of bone abscess in which the colon 
bacillus was present. The patient had been treated with anti-serum and 
also with vaccine. 
CasEe.—JB. coli infection of the urinary ‘tract; treated with anti-coli serum and with 
vaccine. 
