174 Messrs. 8. G. Shattock and L. 8. Dudgeon. [Dee. 21, 
In the next place, the cells from a patient suffering from puerperal fever 
(streptococcal) were substituted for normal washed cells, thus :— 
1 vol. of normal washed cells, 
1,4,  patient’s serum (colon infection), 
1,4, colon bacilli. . 
1 vol. of washed cells of patient suffering from puerperal fever, 
1 ,,  patient’s serum (colon infection), 
1 ,, colon bacilli, 
This observation indicates that, although in colon serum, colon bacilli, 
when presented to normal washed cells, may give a good index (2°3), when 
presented in the same serum to the washed cells of another patient suffering 
from puerperal fever, the index fell to 0°5. 
This can only mean that the cells from the puerperal patient are incapable 
of carrying out the same amount of ingestion as normal cells in the same 
colon serum against the same colon bacilli, ze. the cells cannot make use of 
the phagocytic opportunity offered to them. Their decreased activity may 
be ascribed to damage sustained whilst in the circulating blood, or to the 
exhaustion brought about by the forced production of antibodies under the 
same circumstances. : | | 
It may be inferred from these data that a low index (as reached by 
the usual method) might be found still lower were the patzent’s cells used in 
the patient’s serum, in place of using normal cells in the patient’s serum 
against normal cells in normal serum. 
1 vol. of normal washed cells, 
1 ite Aes os serum, 
1 4, ~~ colon bacilli. 
1 vol. of patient’s washed cells (puerperal fever), 
1,4, normal serum, 
1 ,, ~colon bacilli. 
Indexte ns 0°56. 
This extension of the foregoing observation shows that the cells from the 
same case of puerperal fever do only half the work in normal serum against 
colon bacilli which normal cells do in the same normal serum against colon 
_bacilh. This is the same proportion which was obtained by presenting: 
(1) Normal cells, and 
(2) The same patient’s cells, 
in the patient’s serum, where the patient’s cells are doing only half the 
work of normal cells. 
In the following experiment, colon bacilli were substituted for melanin ; 
