The Influence of Temperature on Phagocytosis. 189 
For 37° ©. this latent period is much shorter. To ascertain this, five- 
minute intervals were taken as in the following experiment :— 
Experiment III. 
Temperature. Incubation period. Cocci per leucocyte. 
37° C. 5 mins. 2°0 
37 ;, 20 W 
37 15 4°3 
37 20 4°0 
37 25 5 °6 
37 30 4 °9 
- 37 35 5°0 
37 40 6°5 
37 45 8 °6 
37 50 12°0 
The five following experiments showing the influence of temperature on 
phagocytosis were carried out in a similar way to Experiment I. Coccal 
emulsions of varying strengths were purposely employed throughout the 
series :— 
Experiment IV. Experiment V. Experiment VI. 
Ped, = 15 mins. J.T. = 15 mins. ft. = 30 mins, 
Temperature. Cocci. Temperature. Cocci. Temperature. Cocci. 
41° C, 17-1 48° ©. 15°7 42° CO. 81 
37 15°3 37 12°3 37 9:0 
17 4°2 31°5 10 °9 30 8°5 
25 °7 4°0 25 6 °3 
20 3 °2 Lire 3°7 
13 0°8 
6°5 ry 
Experiment VII. Experiment VIII. 
iL = 33 mins. poe de ie 30 mms: 
Temperature. Cocci. Temperature. Cocci. 
43° C. 28 °0 41° C, 21 °4 
37 20 °7 36 °6 11°8 
31°5 22 °3 30 8 °2 
25°7 18 *4 Ve 4°8 
20 10 °9 
13 3) 45 
6°5 0°7 
0 ? 
From the results quoted above, it will be apparent that the degree of 
phagocytosis rises, though in a somewhat irregular fashion, as the tempera- 
ture rises, but the results are not sufficiently constant to enable one to 
determine with any accuracy such a value for the temperature-coefficient 
as would allow one to calculate the amount of phagocytosis at a temperature 
A when the amount at a temperature B is known. The strength of the 
* I. T. = Incubation Time. . 
Ohi 
