232 Messrs. Dorée and Gardner. Origin and  [Feb. 10, 
during which the dog had a diet of dog biscuit, but the feces were not 
collected during this interval. During the period it consumed 6758 grammes | 
of meat, or 397 grammes per day. With a view to sweeping out the gut 
at the end, it was fed for two days on bread and bovril; 388 grammes of 
feeces were collected, which on drying at 80° weighed 155 grammes ; 
347 grammes of unsaponifiable matter in the form of a red oil were 
obtained; this was very liquid at 100°, and had a soapy smell. With the 
exception of a little tarry matter this was soluble in alcohol, and, on evapora- 
tion, two crops of crude cholesterol were obtained, weighing respectively 
0°67 and 0:074 gramme. 
This was identified by conversion into the benzoate, and the yields showed 
that it consisted practically entirely of cholesterol. 
The mother liquors, on evaporation, yielded oil, which, on treatment with 
benzoyl chloride in pyridine solution, yielded 0°54 gramme of brown 
erystalline matter which, after recrystallisation from ethyl acetate, melted 
to a turbid liquid at 142° to 145°. This cleared at about 180°, and on 
eooling showed the characteristic colour changes of cholesterol benzoate. 
The total yield of cholesterol was, therefore, approximately 1 gramme, or 
0-06 gramme per day. 
(e) White of Egg, Bread, and Cream.—Immediately after the conclusion 
of the last experiment the animal was put on the above diet. The daily 
ration was prepared by mixing about 150 grammes of bread with the whites 
of three eggs and half a teaspoonful of cream. This was fried and moistened 
with a warm dilute solution of Liebig’s extract of beef. This diet was 
continued for 14 days, during which the animal consumed 2092 grammes 
of bread and the whites of 42 eggs. This proved a very nourishing diet, 
and at the end of the experiment the dog was in excellent condition ; 
130 grammes of faces were passed, and weighed, after drying at 100°, 
35 grammes. On saponification, the soap was white in colour and not 
large in amount. The ethereal solution of the unsaponifiable matter was 
pale yellow, and on ‘evaporation gave 0°59 gramme of a sticky yellow solid. 
This, with the exception of a small amount of tar, was soluble in 90-per-cent. 
alcohol, and on cooling the solution set to a mass of crystals. A small 
portion was examined under the microscope, and appeared to consist of 
small rosettes of needles, but no cholesterol crystals were at first observed. 
On dissolving again on the slide and recrystallising, a small patch of typical 
cholesterol crystals was observed, but the bulk consisted of the rosettes 
referred to. The total weight of crystalline matter was 0°3 gramme. ‘This 
was decolorised by animal charcoal, and crystallised from the least quantity 
of 85-per-cent. alcohol. 
