1908.] Destiny of Cholesterol in the Animal Organism. 233 
The first and main crop of crystals, after drying at 100° C., melted at 
137° to 140°. The acetate, after several recrystallisations, melted at 120° 
to 122°, and no colour changes were noticed. The benzoate began to soften 
at 138°, and melted to a clear liquid at 142° to 143°. On cooling, no colour 
changes could be observed. The substance was probably sitosterol, derived 
from the bread. 
The second and smaller crop from the mother liquors of the above was 
decolorised and recrystallised from alcohol. This was not pure, and the 
quantity was too small for further purification. It began to melt at 120°, 
but the process was not complete at 135°. It was converted into the 
acetate, which after recrystallisation melted sharply at 113° to 114° C. 
This was probably cholesterol acetate, though no colour changes were 
noticed with certainty. The soapy matter left on evaporating the mother 
liquors was treated with benzoyl chloride in pyridine solution, but no 
crystalline matter could be obtained. In this experiment, therefore, only 
traces of cholesterol were obtained. 
(f) Oatmeal Porridge.—As the dog at the conclusion of experiment (e) was’ 
in excellent health, it was at once put on a diet of porridge made from 
coarse oatmeal and water with a little salt. This diet was continued 
for 31 days, during which the dog consumed 2870 grammes of oatmeal ; 
' 212 grammes of dried faeces were obtained. The ethereal extract, measuring 
about 1°5 to 2 litres, on saponification set to an almost solid mass of soap. 
This, however, filtered easily, and was readily washed. The unsaponifiable 
matter was a reddish brown oil, weighing 2°7 grammes. With the exception 
of 0°27 gramme of tarry matter this was soluble in alcohol. On standing, 
the solution deposited a slimy brown mud. This was taken up in ethyl 
acetate, and, on standing, the solution deposited several crops of white needle- 
shaped crystals, the total weight being 0°255 gramme. The ethyl acetate 
mother liquors, on spontaneous evaporation, yielded an oil. 
The needle-shaped crystals were readily soluble in petrol and in alcohol, 
but did not crystallise readily from these solvents. After several recrystal- 
lisations from ethyl acetate the substance was obtained in the form of long, 
flat, slender needles, which melted sharply at 133° to 134°C. The acetate 
began to soften at 70°, and melted to a clear liquid at 98° C., but we had 
not enough for a satisfactory purification. It was evidently the same as 
the phytosterol obtained in experiment (a). The alcoholic mother liquors 
_ from the above-mentioned muddy solid, on spontaneous evaporation, gave 
an oil through which crystalline matter was disseminated. This was 
recrystallised from 85-per-cent. alcohol when 0:172 gramme of whitish 
crystals was deposited. Under the microscope these were found to consist 
