234 Messrs. Dorée and Gardner. Origin and  [Feb. 10, 
of long hexagonal plates, and no typical cholesterol crystals were observed. 
After recrystallismg from absolute alcohol until quite white, the substance 
melted at 124° to 125°. The acetate crystallised in microscopic plates, 
which melted at 118° to 122°. These properties agree with those of the 
para-sitosterol of Burian. 
All the mother liquors, from which the above-mentioned crystalline 
substances were obtained on spontaneous evaporation yielded viscid oils, 
which really constituted the bulk of the unsaponifiable matter. In order to 
ascertain whether any cholesterol or other crystalline substances were held in 
solution in these oils, they were dissolved in pyridine and treated with benzoyl 
chloride. After standing, the solutions were poured into water and the slimy 
deposits dissolved in hot absolute alcohol and left to crystallise. One oil 
yielded 0:094 gramme and another 0°04 gramme of crystalline matter, 
difficultly soluble in absolute alcohol. On purification, this was obtained in 
the form of glistening plates which melted at 145° to a turbid liquid. This: 
cleared at about 175° and on cooling showed the colour changes characteristic 
of cholesterol benzoate. The mother liquors, on spontaneous evaporation, 
dried to a viscid oil, which, on long standing, showed no sign of crystallising. 
Only about 0-1 gramme of cholesterol was therefore obtained in the month, 
or 6:003 gramme per day. 
(2) Rice, Gelatine, and Butter.—After experiment (f) the dog was fed for four 
or five days on dog biscuit and porridge and given regular exercise, and as it 
" appeared to be in good health, the final experiment was proceeded with. Each 
day’s ration consisted of 114 grammes of rice, which was well boiled and mixed 
with 114 grammes of a 10-per-cent solution of gelatine and 28 grammes of 
butter. The mass was flavoured with a dilute solution of Liebig’s extract of 
meat. During 18 days the dog consumed 2052 grammes of rice, about 205: 
grammes of gelatine, and 5V4 grammes of butter. The weight of dried faces: 
was 115 grammes. 
1:316 grammes of unsaponifiable matter was obtained as a vaseline-like oil.. 
This, with the exception of 0°06 gramme of tarry substance, dissolved in 50 c.c. 
of hot 90-per-cent. alcohol. Three crops of impure crystalline matter were: 
obtained, (1) 0°37 gramme; (II) 0°16 gramme; and (III) 0:14 gramme. The 
mother liquors dried to an oil, which, on long standing, showed no sign of 
erystallising. Crop I, after decolorising and recrystallising from 90-per-cent. 
alcohol, was obtained in the form of white crystals not unlike cholesterol 
in appearance. On heating, these began to soften at 120° and melted at about. 
129°. A microscopic examination showed that the substance was a mixture. 
We tried to separate the substances by means of the benzoates. The crude 
benzoate obtained by the pyridine method crystallised from alcohol, apparently 
