1908.] Destiny of Cholesterol in the Animal Organism. 235 
in two forms, one light and floating in the liquid and the other granular 
and adhering to the sides of the beaker. These were separated as far as 
possible and recrystallised separately. The light form, after recrystallisation 
from alcohol, in which it was sparingly soluble in the cold, melted at 139° to 
a pale brownish liquid which was slightly turbid, but not so markedly as is 
usually the case with cholesterol benzoate and did not become any clearer 
at 170° to 180°. On cooling, no colour changes were observed at the higher 
temperature, but at the point of solidification a transient lilac blue appeared, 
but was not well marked. This substance might have been impure cholesterol 
benzoate, but we could not be certain. 
The granular portion, which was the greater quantity, after recrystallisation 
shrank together at 138° C. and melted sharply at 139° C. to a perfectly clear 
liquid. It showed no colour changes and was not cholesterol benzoate. 
Crop II was decolorised by charcoal and recrystallised from alcohol. It 
began to soften at 120° and melted at 135°. It was mostly cholesterol, for 
the benzoate made by the pyridine method melted at 140° to 143° to a turbid 
liquid which went clear at 178° and on cooling showed the colour changes in 
a well-marked manner. 
Crop III was very impure and greasy. It was therefore treated at once 
with pyridine and benzoyl chloride. A minute amount of crystalline matter 
was obtained which melted at 145° to a turbid liquid and appeared to clear 
at 178°. It was, however, yellow in colour, and we could not observe the 
colour changes. The oils from the mother liquors were benzoylated, but no 
crystalline matter was obtained. In this experiment, therefore, 0°667 gramme 
of impure crystalline matter was obtained. This contained some cholesterol, 
but we could not determine the amount. We do not think that more than 
one-third of it was cholesterol, certainly not one-half. The rest evidently 
consisted of phytosterols from the rice. 
Discussion of Lesults, 
Different observers have given very variable figures for the daily quantity 
_ of bile secreted by the dog, as the following table shows :— 
Quantity of bile in grammes secreted 
per kilogramme-weight 
Observer. in 24 hours. 
Hriedlander and Barisch® *......:....... 19°9 
Biddercamd Schmidt ......csc.ccc-+ ccs -0< 24°5 
Ietoe ninadmie No... c ss cod deeeeeecwec dees. Bh 
era Ce The... Sea caadt eas mavens 10:0 
VOL. LXXX.—B. E 
