Studies on Enzyine Action. 317 
Table ILI. 
Cane-sugar. Raffinose (commercial). 
342 grammes of cane-sugar (1/10 mol. 59°4 grammes of raffinose (1/10 mol. 
Fane, C,.H..0};) +4 ¢.c. of strong invertase ©gH3,0)5.5H,0) +4 ¢.c. of strong 
extract per 1000 c.c. invertase extract per 1000 c.c. 
Per cent. Per cent. 
“D- hydrolysed. ee = hydrolysed. x 
mins. 
0 4°36 0°0 = 12°25 0°0 a 
5 3°88 8°3 753 12°15 1°8 157 
15 2°87 25 ‘9 868 11 °92 5°9 176 
25 2°09 39 °5 865 11°51 13 “+ 249 
40 0°78 62 *4 1062 11°14 20°1 243 
60 —0°13 78 2 1102 10 °63 29°3 251 
95 —0°87 oe 1106 9 95 41 °6 246 
140 —1 02 93 °7 859 9°30 53 °*4 237 
200 —1°10 955 656 8°57 66 °6 238 
260 —1°15 96 ‘0 537 7°95 EES 252 
x —1°38 100 ‘0 — 6°73 100 °0 — 
On plotting curves to represent the rates at which the two sugars are 
changed, it is seen that whilst one-half of the cane-sugar is hydrolysed in 
33 minutes, the half of the raffinose is hydrolysed only after the lapse of 
Table III. 
| Cane-sugar. Raffinose (recrystallised). 
34:2 grammes of cane-sugar (1/10 mol. 59°4 grammes of raffinose (1/10 mol. 
: C).H..0;,) + 4 ¢.c. of strong invertase C1gH30)¢.5H.O) + 4 ¢.c. of stron 
Time Lait oa 8 Odean ce 8 
Ba «il extract per 1000 c.c. invertase extract per 1000 c.c. 
aro: Per cent. ayo: Per cent. 
7 hydrolysed. Be ‘ hydrolysed. eS 
mins, 
0 4 504 0°0 — 13 768 0-0 — 
10 3 °332 17° 829 13 °462 4 °6 203 
20 2 °100 35 °6 957 13-168 9-0 204: 
30 1-074 50 ‘9 1043 12 ‘918 127 200 
40 0 °306 62 °3 1058 12 668 16 °4 195 
60 —0 848 79 °A 1143 — — — 
80 —1 °490 88 °9 1193 11 814 29 °2 187 
120 —1°810 93 ‘6 997 11 044 40 °6 189 
160 —1 982 96 °2 888 10 °398 50 °3 190 
200 —2:°114 98 *2 868 - 9 °850 58 °5 191 
x — 2 *238 100 ‘0 — 7 ‘065 100 ‘0 = 
