1908. | | Studies on Enzyme Action. a3] 
The following values were obtained in an experiment in which the 
precipitation method was used in determining the cyanide :— 
Time. | Glucose. | HCN. 
hours. 
2 38 °3 31-0 
3 48 -O 41 °O 
A, 5A 4 49 °5 
5 60 °8 5a 7a 
6 65 °6 61 °25 
24 94, °5 93 0 
An emulsin which had been heated during three hours at 55°, although 
weaker, produced a smaller proportion of cyanide as compared with that of 
glucose than the unheated extract— 
Time. Glucose. HCN. 
hours. 
17°35 77°6 68 °8 
40 °5 89 -O TA5 
The amount of Fischer’s glucoside we have separated from partially 
hydrolysed material has been small—at most 3 or 4 per cent. of the weight 
of the amygdalin used; apparently there is no great difference between the 
rates at which this glucoside and amygdalin are hydrolysed by the 8-glucase. 
In some cases we have even failed to isolate a crystalline product and have 
had reason to suspect that the glucoside has been in part converted during 
its manipulation into the stereoisomeride. 
La 
