1908.] On the Experimental Treatment of Trypanosomiasis. 487 
Experiments made with Rats treated with Antimony, in order to find out in 
7 | what Organs the Trypanosomes are latent. 
The following initial experiments were made in order to find out where 
the trypanosomes rest during the period in which the peripheral blood is 
free from them, after treatment with antimony. Further experiments are 
being carried on for the purpose of ascertaining in what forms they are 
present in the organs of treated animals. 
The rats were inoculated with Nagana, which is less affected by antimony 
than Surra, and were all treated with four doses of sodium antimony tartrate. 
The rats were killed at various intervals, and the organs selected (the liver 
and bone-marrow) were made into an emulsion with a minimum quantity of 
0°75 per cent. salt-solution, and injected into other rats in doses of 1 c.c.; the 
same dose of blood from the heart was also given. 
In the following table the signs + and — are used respectively to denote 
a positive or negative result. 
Number of days after | 
No. | treatment upon which | Blood. Liver. Marrow. 
rats were killed. 
FOOON OOP CDE - 
aN 
ee PM ee Fs (FE Mas [ise | 
fie et eee sh pee ele | 
+t+eet1 +1 i 
ft 
ho 
S 
From this table it would appear that the bone-marrow is the place where 
the trypanosomes can live longest, and that the liver is also a place where 
they can find protection. This is borne out by some experiments we have 
made upon trypanosomiasis in birds, in which cultivations of trypanosomes 
can often be made from the bone-marrow when they cannot be made either 
from the organs or the blood. The doses given to the above rats were rather 
‘under those which we should judge to be curative, but in four cases the 
results were entirely negative. | 
