Doe Mr, L. 8..Dudgeon. On the Presence of [July 31, 
Nature of Cases investigated. — 
Every care has been taken to make the classification of the various 
diseases which have been investigated accurate. Wherever it is permissible, 
the various groups and sub-groups are arranged as the result of a combina- 
tion of pathological, bacteriological, and clinical investigations* :— 
Group I.—10 cases of acute pneumonia and acute empyema. 
Group II.—22 cases of tuberculosis (mostly acute pulmonary), and 1 case 
of leprosy. 
Group II[—12 cases of epilepsy. 
Group IV.—5 cases of acute peritonitis due to appendicitis. 
Group V.—d cases of infections due to the bacilli of the typhoid and para- 
typhoid family. 
Group VI—26 cases of anemia, including 14 of pernicious anemia, 
chlorosis, lymphzmia, myelemia, congenital cholemia, and examples of 
anemia secondary to various well recognised conditions. 
Group VII.—7 cases of infections due to the streptococci. 
Group VIII.—3 cases of infections due to the Staphylococcus pyogenes 
aureus. 
Group IX.—Miscellaneous cases. Infective endocarditis, purpura, jaundice, 
chronic lead poisoning, chronic renal disease, coal gas poisoning, acute 
poisoning of unknown origin, eclampsia. 
Heem-ayglutinins. 
Technique-—The blood was collected for these experiments in 0°85-per-cent. 
pure sodium chloride, and 0°85-per-cent. pure sodium citrate in distilled 
water. The corpuscles were then centrifuged and thoroughly washed free 
from plasma in sodium chloride. A 5-per-cent. suspension of the washed 
corpuscles was made in normal saline. The blood was also collected in glass 
tubes and centrifuged in the course of an hour or so when clotting had 
completely taken place, as by this means a serum was obtained showing no 
red tinge. 
In experiments on hem-agglutinins, hem-opsonins, and hemo-lysins, the 
investigations were made immediately the blood was withdrawn. 
For the agglutinative tests, one measured volume of the 5-per-cent. 
solution of washed red blood corpuscles was drawn into a capillary tube with 
an equal volume of blood serum. These were then thoroughly mixed and 
* Tam greatly indebted to one of the workers in my laboratories, Mr. H. A. F. Wilson, 
for much valuable help in obtaining samples of the blood from the cases which I was 
investigating. 
